ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
M 2.47 or less F 0.13-9.88 (ng/mL)
IGF-1 (Somatomedin C)
Serum
0.5
S09 ↓ A00
(7 days)
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
See below
Prolactin
Serum
0.4
S09 ↓ A00
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
M 4.29-13.69 Premenopausal women 4.91-29.32 Postmenopausal women 3.12-15.39 (ng/mL)
Adrenocorticotropic hormone (ACTH)
Plasma
0.5
PN2,PN5 ↓ A00
(21 days)
2-4
ECLIA
ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
7:00 a.m.-10:00 a.m. 7.2-63.3 (pg/mL)
Luteinizing hormone (LH)
Serum
0.5
S09 ↓ A00
2-4
CLIA
CLIA (Chemiluminescent immunoassay) A method in which after an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, in which the luminescence intensity of a chemiluminescent substrate is measured.
See below
Follicle stimulating hormone (FSH)
Serum
0.5
S09 ↓ A00
2-4
CLIA
CLIA (Chemiluminescent immunoassay) A method in which after an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, in which the luminescence intensity of a chemiluminescent substrate is measured.
See below
Thyroid-stimulating hormone (TSH) 〔CLEIA〕 IFCC
Serum
0.5
S09 ↓ A00
(21 days)
2-4
CLEIA
CLEIA (Chemiluminescent enzyme immunoassay) A method in which an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, a chemiluminescent substrate is added and the luminescence intensity is measured.
0.61-4.23(μIU/mL)
Arginine vasopressin(AVP)
Plasma
1.5
PN7 ↓ A00
5-7
RIA2 antibody method
RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.
Water limit: 4.0 or less Free drinking water: 2.8 or less (pg/mL)
IRMA (Immuno radio metric assay) Immuno radiometric assay One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody. This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.
ECLIA (Electro chemiluminescence immunoassay) Electrochemiluminescence immunoassay A method to measure the luminescence intensity of ruthenium pyridine complexes by electrochemical reaction, in which antibody-conjugated beads are used to react with antigens, followed by a secondary reaction of antibodies labeled with ruthenium pyridine complexes with the antigens.
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