Amino acid analysis(41 types)

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biochemical test
low molecular nitrogen compounds
Amino acid analysis(41 types)

Laboratory：Hachioji

○Amino acid analysis(41 types)

CODE：0135 7

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Amino acid analysis(41 types)
acidic urine storage
2.0
U00
（21 days）
7-10 HPLC
HPLC (High performance liquid chromatography) Liquid chromatography
Method for high-speed and high-precision separation using liquid chromatography employing a mobile phase with liquid and employing high-density packing columns and high-pressure pumps
See below

COMMENT

Keep your urine for 24 hours in a urine collection bottle containing approximately 20mL of 6N hydrochloric acid, mix well, and be sure to freeze the required amount before submitting.
If the amount of urine is small, add 1 mL of 6N hydrochloric acid to 100 mL of urine.
Please be sure to store urine in an acidic manner (pH 1.0 to 3.0).

CONTAINER

U00 旧容器記号 Y

尿用容器

貯蔵方法：室温

supplementary information

[0951, 0089, 0954] アミノ酸分析 (39種類 (血漿)、41種類 (尿)、9種類 (血漿)) 基準値

単位　血漿：nmol/mL、尿：μmol/day (ただし◎印 (Urea) はmmol/day)
アミノ酸名	略号	基準値
アミノ酸名	略号	血漿	尿
Taurine	Tau	39.5～93.2	322.2～5214.5
Phosphoethanolamine★	PEA		31.0～110.0
Urea★	Urea		◎130.3～493.2
Aspartic acid	Asp	2.4以下	12.7以下
Hydroxyproline	Hypro	21.6以下	ND
Threonine	Thr	66.5～188.9	79.9～528.3
Serine	Ser	72.4～164.5	208.8～1020.0
Asparagine	Asn	44.7～96.8	60.7～372.3
Glutamic acid	Glu	12.6～62.5	11.3～42.7
Glutamine	Gln	422.1～703.8	207.0～1357.3
Sarcosine	Sarco	TR	99.0以下
α-Aminoadipic acid	α-AAA	ND	16.7～118.6
Proline	Pro	77.8～272.7	ND
Glycine	Gly	151.0～351.0	652.1～3670.6
Alanine	Ala	208.7～522.7	141.2～833.9
Citrulline	Cit	17.1～42.6	13.5～55.6
α-Aminobutyric acid	α-ABA	7.9～26.6	27.1以下
※Valine	Val	147.8～307.0	24.8～82.2
Cystine	Cys	13.7～28.3	23.7～170.9
Cystathionine	Cysthio	TR	TR～44.7
※Methionine	Met	18.9～40.5	TR～20.2
※Isoleucine	Ileu	43.0～112.8	7.5～23.5
※Leucine	Leu	76.6～171.3	24.6～89.3
※Tyrosine	Tyr	40.4～90.3	50.6～308.4
※Phenylalanine	Phe	42.6～75.7	27.2～110.2
γ-Amino β-hydroxy butyric acid	γ-A β-HBA	ND	ND
β-Alanine	β-Ala	TR	TR～153.0
β-Amino-iso-butyric acid	β-AIBA	TR	TR～1623.9
γ-Aminobutyric acid	γ-ABA	ND	ND
Monoethanolamine★	MEA	10.4以下	195.3～606.2
Homocystine	Homocys	ND	ND
※Histidine	His	59.0～92.0	436.4～2786.5
3-Methylhistidine	3-Me His	5.0以下	113.4～480.9
1-Methylhistidine	1-Me His	18.5以下	59.3～2816.2
Carnosine★	Carno	ND	87.6以下
Anserine★	Ans	ND	231.4以下
Tryptophan	Trp	37.0～74.9	20.7～150.7
Hydroxylysine	Hylys	ND	22.9以下
Ornithine	Orni	31.3～104.7	6.9～43.9
※Lysine	Lys	108.7～242.2	51.6～1639.6
※Arginine	Arg	53.6～133.6	11.6～54.8
Total AA (総アミノ酸：非必須アミノ酸の和+必須アミノ酸の和)		2068.2～3510.3
NEAA (非必須アミノ酸の和)		1381.6～2379.4
EAA (必須アミノ酸の和)		660.0～1222.3
BCAA (分岐鎖アミノ酸の和)		265.8～579.1
EAA/NEAA (必須アミノ酸の和/非必須アミノ酸の和)		0.40～0.63
BCAA/Total AA (分岐鎖アミノ酸の和/総アミノ酸)		0.11～0.18
フィッシャー比		2.43～4.40

※印はアミノ酸分析 (9種類) の実施アミノ酸名
ND：測定感度以下　TR：痕跡
★印は「アミノ酸」ではありませんが、本項目に含めて測定いたします。
EAA/NEAA、BCAA/Total AA、フィッシャー比においては単位なし

Amino acid analysis (39 types (plasma), 41 types (urine), 9 types (plasma)) reference value

Units: nmol/mL for plasma, μmol/day for urine (mmol/day for ◎[Urea])
Amino acid name	Abbreviation	Reference range
Amino acid name	Abbreviation	Plasma	Urine
Taurine	Tau	39.5 – 93.2	322.2 – 5214.5
Phosphoethanolamine★	PEA		31.0 – 110.0
Urea★	Urea		◎130.3 – 493.2
Aspartic acid	Asp	≤ 2.4	≤ 12.7
Hydroxyproline	Hypro	≤ 21.6	ND
Threonine	Thr	66.5 – 188.9	79.9 – 528.3
Serine	Ser	72.4 – 164.5	208.8 – 1020.0
Asparagine	Asn	44.7 – 96.8	60.7 – 372.3
Glutamic acid	Glu	12.6 – 62.5	11.3 – 42.7
Glutamine	Gln	422.1 – 703.8	207.0 – 1357.3
Sarcosine	Sarco	TR	≤ 99.0
α-Aminoadipic acid	α-AAA	ND	16.7 – 118.6
Proline	Pro	77.8 – 272.7	ND
Glycine	Gly	151.0 – 351.0	652.1 – 3670.6
Alanine	Ala	208.7 – 522.7	141.2 – 833.9
Citrulline	Cit	17.1 – 42.6	13.5 – 55.6
α-Aminobutyric acid	α-ABA	7.9 – 26.6	≤ 27.1
*Valine	Val	147.8 – 307.0	24.8 – 82.2
Cystine	Cys	13.7 – 28.3	23.7 – 170.9
Cystathionine	Cysthio	TR	TR – 44.7
*Methionine	Met	18.9 – 40.5	TR – 20.2
*Isoleucine	Ileu	43.0 – 112.8	7.5 – 23.5
*Leucine	Leu	76.6 – 171.3	24.6 – 89.3
*Tyrosine	Tyr	40.4 – 90.3	50.6 – 308.4
*Phenylalanine	Phe	42.6 – 75.7	27.2 – 110.2
γ-Amino β-hydroxy butyric acid	γ-A β-HBA	ND	ND
β-Alanine	β-Ala	TR	TR – 153.0
β-Amino-iso-butyric acid	β-AIBA	TR	TR – 1623.9
γ-Aminobutyric acid	γ-ABA	ND	ND
Monoethanolamine★	MEA	≤ 10.4	195.3 – 606.2
Homocystine	Homocys	ND	ND
*Histidine	His	59.0 – 92.0	436.4 – 2786.5
3-Methylhistidine	3-Me His	≤ 5.0	113.4 – 480.9
1-Methylhistidine	1-Me His	≤ 18.5	59.3 – 2816.2
Carnosine★	Carno	ND	≤ 87.6
Anserine★	Ans	ND	≤ 231.4
Tryptophan	Trp	37.0 – 74.9	20.7 – 150.7
Hydroxylysine	Hylys	ND	≤ 22.9
Ornithine	Orni	31.3 – 104.7	6.9 – 43.9
*Lysine	Lys	108.7 – 242.2	51.6 – 1639.6
*Arginine	Arg	53.6 – 133.6	11.6 – 54.8
Total AA (total amino acids; non-essential amino acids + essential amino acids)		2068.2 – 3510.3
NEAA (non-essential amino acids)		1381.6 – 2379.4
EAA (essential amino acids)		660.0 – 1222.3
BCAA (branched chain amino acids)		265.8 – 579.1
EAA/NEAA (essential amino acids/non-essential amino acids)		0.40 – 0.63
BCAA/Total AA (branched chain amino acids/total amino acids)		0.11 – 0.18
Fisher ratio		2.43 – 4.40

The mark* indicates the name of amino acid for the amino acid analysis (9 types)
ND: Not detectable (Below measurement sensitivity)
TR: Trace
Substances with the mark ★ are not amino acids, but measured in the respective item.
No units for EAA/NEAA, BCAA/Total AA, and Fisher ratio

TEST DIRECTORY