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Laboratory:Hachioji

Estriol (E3)(Suspended beyond orders placed 11-27-2019)

  • TEST NAME SPECIMEN
    REQUIREMENT
    (mL)
    CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)
    TURNAROUND
    TIME (DAY)
    METHODOLOGY REFERENCE RANGE
    (UNIT)
  • INACTIVE
    Estriol (E3)(Suspended beyond orders placed 11-27-2019)
    urine collected for 24 hrs
    5
    U00 Freeze
    8-14 RIA ammonium sulfate precipitation method

    Radio immunoassay (RIA)
    A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity.
     To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.

    See below.

COMMENT


Specify the sex of the person.
If pregnant, specify the week of pregnancy.

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