CD4 | TEST DIRECTORY

Laboratory：Hachioji

○CD4

CODE：5002 4

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
CD4
Blood (heparin added)
3
PH5
3-5 Flow cytometry(FCM)
Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
25.0～54.0(%)

COMMENT

Acceptable days are Monday to Friday.
Please see below for the list of implementation items.
If you request 10 or more items, please collect 5.0mL of blood.
About requesting a cell-mediated immunity test
1. Please submit the specimen on the same day after collection.
2. If there are few lymphocytes, please collect more blood.
3. If requesting bone marrow fluid, please use a special container (H00) and submit it refrigerated. However, for CD34 quantitative, if it is diluted with storage solution, correct test results will not be obtained, so please use a special container (PH5).
4. If you are making a request for a tissue such as lymph node, please suspend it in a special container (H20) and submit it in a refrigerator.
Collect blood into the container shown below, mix well, and store at room temperature.
(For 10 or more items, please collect 5.0mL of blood.)
After collecting the specimen, please submit it on the same day.

CONTAINER

PH5 旧容器記号 G 12

ヘパリン入り (真空採血量5mL)

内容：ヘパリンNa　65IU
貯蔵方法：室温
有効期間：製造から2年

supplementary information

モノクローナル抗体によるリンパ球表面マーカーの自動解析実施項目一覧

CD : Cluster of differentiation
項目コード新項目コード	CD No. JLAC10	基準値 (単位)	実施料判断料	主として反応する細胞・代表的な反応性
5005 6 3946 8 017504401 352401 3524 3205	CD1a 5I060-0000-019-662	1.0 以下 (%)		胸腺皮質細胞
5006 3 3945 9 017514401 352601 3526 3206	CD2 5I064-0000-019-662	71.0～91.0 (%)		T細胞・Eロゼット形成細胞
5001 7 3972 9 017464401 352801 3528 3207	CD3 5I067-0000-019-662	58.0～84.0 (%)	190 ※6	成熟 (末梢) T細胞
5002 4 3973 8 017474401 353001 3530 3208	CD4 5I070-0000-019-662	25.0～54.0 (%)		ヘルパーT細胞
5003 1 3974 7 017484401 353601 3536 3209	CD8 5I082-0000-019-662	23.0～56.0 (%)		細胞傷害性T細胞
3210	CD4/CD8 比	0.40～2.30
5039 1 3956 7 017594401 353201 3532 3211	CD5 5I073-0000-019-662	60.0～90.0 (%)		T細胞・一部のB細胞
0011 3 3964 8 010024401 353301 3533 3212	CD7 5I079-0000-019-662	72.0～90.0 (%)		T細胞
5053 9 3957 6 017674401 353701 3537 3213	CD10 5I088-0000-019-662	1.0 以下 (%)		ALL・好中球
5011 2 3976 5 017534401 366301 3663 3228	CD11b 5I092-0000-019-662	18.0～49.0 (%)		NK細胞・T細胞の一部
0021 9 3948 6 010094401 354001 3540 3229	CD13 5I098-0000-019-662	9.0 以下 (%)		単球・顆粒球・骨髄細胞
0022 6 3952 1 010104401 354101 3541 3231	CD14 5I101-0000-019-662	5.0 以下 (%)		単球
5031 3 3958 5 017584401 354301 3543 3232	CD16 5I110-0000-019-662	6.0～39.0 (%)		NK細胞 (Fcγレセプター)・好中球
0023 3 3959 4 010114401 354401 3544 3214	CD19 5I119-0000-019-662	5.0～24.0 (%)		B細胞
5359 3 3947 7 018294401 354501 3545 3215	CD20 5I122-0000-019-662	3.0～20.0 (%)		成熟B細胞
0036 1 3966 6 010184401 354601 3546 3216	CD21 5I125-0000-019-662	3.0～18.0 (%)		成熟B細胞
0494 3 3898 2 012474401 354701 3547 1208	CD22 5I128-0000-019-662	2.0～17.0 (%)		B細胞
5360 3 3960 2 018304401 354901 3549 3234	CD25 5I137-0000-019-662	8.0～34.0 (%)		IL-2レセプター Tac
0495 1 3900 8 012484401 355001 3550	CD30 5I152-0000-019-662	1.0 以下 (%)		活性化T・B細胞・ホジキン細胞
0169 2 3967 5 011074401 355101 3551 3235	CD33 5I161-0000-019-662	5.0 以下 (%)		顆粒球・単球・骨髄細胞
0497 5 3899 1 012494401 355201 3552 3217	CD34 5I164-0000-019-662	1.0 以下 (%)		造血幹細胞
5008 8 3977 4 017524401 355501 3555 3237	CD38 5I176-0000-019-662	18.0～59.0 (%)		活性化および増殖細胞・形質細胞 (強陽性)
0184 7 3968 4 011204401 355601 3556 3239	CD56 5I238-0000-019-662	9.0～43.0 (%)		NK細胞
5030 6 3961 1 017574401 355701 3557 3238	CD57 5I241-0000-019-662	4.0～45.0 (%)		NK細胞・LGL
5004 9 3979 2 017494401 364301 3643 1910	HLA-DR 5I680-0000-019-662	11.0～46.0 (%)		B細胞・活性化T細胞 (HLA-DR抗原)・単球

Item List of Lymphocyte surface marker automate analysis by monoclonal antibodies

CD : Cluster of differentiation
CODE	CD No.	Reference range (unit)	Mainly reacting cells/representative reactivity
5005 6	CD1	≤ 1.0 (%)	thymus-derived lymphocyte
5006 3	CD2	71.0 – 91.0 (%)	Pan T cells, E rosette-forming cells
5001 7	CD3	58.0 – 84.0 (%)	Mature (peripheral) T cells
5002 4	CD4	25.0 – 54.0 (%)	Inducer T cells, T helper cells
5003 1	CD8	23.0 – 56.0 (%)	Suppressor/cytotoxic T cells
	CD4/CD8 ratio	0.40 – 2.30
5039 1	CD5	60.0 – 90.0 (%)	Pan T cells, some B cells
0011 3	CD7	72.0 – 90.0 (%)	Pan T cells
5053 9	CD10	≤ 1.0 (%)	Common-ALL, neutrophils
5011 2	CD11b	18.0 – 49.0 (%)	Some NK cells, some suppressor T cells
0021 9	CD13	≤ 9.0 (%)	Monocytes, granulocytes, bone marrow cells
0022 6	CD14	≤ 5.0 (%)	Monocytes
5031 3	CD16	6.0 – 39.0 (%)	NK cells (Fcγ receptor)
0023 3	CD19	5.0 – 24.0 (%)	Pan B cells
5359 3	CD20	3.0 – 20.0 (%)	Pan B cells
0036 1	CD21	3.0 – 18.0 (%)	C3d/EBV receptor, mature B cells
0494 3	CD22	2.0 – 17.0 (%)	Mature B cells
5360 3	CD25	8.0 – 34.0 (%)	p55 Tac IL-2 receptor
0495 1	CD30	≤ 1.0 (%)	Activated T cells, activated B cells, Hodgkin cells
0169 2	CD33	≤ 5.0 (%)	Immature granulocytes: APL, AML
0497 5	CD34	≤ 1.0 (%)	Bone marrow stem cells, precursor B cells
5008 8	CD38	18.0 – 59.0 (%)	Activated and proliferating cells
0184 7	CD56	9.0 – 43.0 (%)	Neural cell adhesion molecule (NCAM) , NK cells, LAK cells
5030 6	CD57	4.0 – 45.0 (%)	Some NK cells, some suppressor T cells
5004 9	HLA-DR	11.0 – 46.0 (%)	B cells, activated T cells (HLA-DR antigen)

TEST DIRECTORY

○CD4

COMMENT

CONTAINER

PH5 旧容器記号 G 12

PH5 Previous container symbol G

supplementary information

モノクローナル抗体によるリンパ球表面マーカーの自動解析実施項目一覧

Item List of Lymphocyte surface marker automate analysis by monoclonal antibodies

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