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Laboratory：Hachioji

○Drug-induced lymphocyte stimulation test (DLST)

CODE：0517 5

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • Specified days
    Drug-induced lymphocyte stimulation test (DLST)
    Blood (heparin added)
    1 medical drug 12.0
    PH9 Room temperature
     8-10 3H-thymidine uptake ability

    3H-thymidine uptake capacity (3H-TdR uptake)
    A method using the phenomenon that lymphocytes become blastic in response to stimulation by non-self antigens.
    Lymphocytes are cultured with the addition of the stimulants and 3H-thymidine, and the amount of 3H-thymidine incorporated into the cells by DNA synthesis is measured as radioactivity. PHA, ConA, and drugs are used for stimulants.

     Negative

COMMENT


Acceptable days are Monday to Friday. 5.0mL of blood is required for each additional drug. Please submit your requested medications along with your blood. (As a general rule, intradermal reaction injections cannot be tested.) If you suspect that heparin is the causative agent, use (PNK) containers for all blood collection tubes.
About specimens for drug-induced lymphocyte stimulation test (DLST)
1. For each increase in drug 5.0mL of blood is required.
2. One drug requires 5 million lymphocytes. In some cases, it may not be possible to obtain the required number of lymphocytes for the test, making the test impossible.
3. As a guideline, if your white blood cell count is 3000mm3or less, please draw twice the amount of blood and submit it. If you know the detailed white blood cell count and lymphocyte percentage, please contact your sales representative.
4. If you are requesting two or more items, please be sure to indicate the order of priority.
5. Please submit the requested medication along with the blood.
Tablet (1 tablet), Capsule (1 capsule), Powder (about 1 package per dose), Liquid type oral medicine (about 0.5mL), Injection (1　vial【ampoule】). Test ampoules for intradermal reactions cannot be tested due to the small amount. If you would like to request testing for other drugs, please contact your sales representative.
6. If you suspect that heparin is the causative drug, please collect all blood in a special container (PNK) and submit it.
After collecting the specimen, please submit it on the same day.

CONTAINER



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