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biochemical test
proteins, serum colloid reaction

Laboratory：Hachioji

○proteins, serum colloid reaction

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Protein, total (TP)	serum 0.5	S09 ↓ A00		（1 month）	2-4	Biuret method	6.7-8.3 (g/dL)
Protein, total (TP)	CSF 0.5	A00			2-4	Pyrogallolred method	15-45 (mg/dL)
Protein, total (TP)	urine collected for 24 hrs 0.5	U00			2-4	Pyrogallolred method	31.2-120.0 (mg/day)
Albumin,quantitative	serum 0.5	S09 ↓ A00		（1 month）	2-4	Nephelometry Nephelometry A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.	3900-4900 (mg/dL)
Albumin (Alb)	serum 0.5	S09 ↓ A00		（21 days）	2-4	Improved BCP method	3.8-5.2 (g/dL)
Albumin, Urine	urine collected for 24 hrs 0.5	U00		（1 month）	2-4	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	2-20 (mg/day)
Albumin, Urine (creatinine ratio)	random urine 1	U00		（1 month）	2-4	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	Urinary albumin ratio ≦ 10.0 (mg/g CRE)
A/G ratio	serum 0.5	S09 ↓ A00		（1 month）	2-4	Improved BCP method/Biuret method	1.1-2.1
Protein fractionation	serum 0.3	S09 ↓ A00			2-4	Capillary electrophoresis Electrophoresis A technique for analysis of macromolecules based on the following phenomena: When an electric current is applied to an electrolyte solution in which charged particles suspended, the particles migrate towards the electrode of opposite charge. Based on the length of migration, the target molecule is determined. Cellulose acetate membrane, agarose gel, and polyacrylamide gel are used as support media.	ALB 55.8-66.1 (%) α1-globulin 2.9-4.9 (%) α2-globulin 7.1-11.8 (%) β1-globulin 4.7-7.2 (%) β2-globulin 3.2-6.5 (%) γ-globulin 11.1-18.8 (%) A/G 1.3-1.9
Urine protein fractionation	random urine 1	U00			2-4	Agarose gel electrophoresis Electrophoresis A technique for analysis of macromolecules based on the following phenomena: When an electric current is applied to an electrolyte solution in which charged particles suspended, the particles migrate towards the electrode of opposite charge. Based on the length of migration, the target molecule is determined. Cellulose acetate membrane, agarose gel, and polyacrylamide gel are used as support media.	(%)
Zinc sulfate turbidity test (ZTT, Kunkel)	serum 0.5	S09 ↓ A00		（3 days）	2-4	A modified standard method by theJapanese Society ofGastroenterology’sWorking Group onLiver Function	4.0-12.0 (U)