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endocrinology test
hypothalamus hormones and pituitary hormones

Laboratory：Hachioji

○hypothalamus hormones and pituitary hormones

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Growth hormone (GH)	serum 0.5	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	Male: ≦ 2.47 Female: 0.13-9.88 (ng/mL)
IGF-I (Somatomedin C)	serum 0.3	S09 ↓ A00		（21 days）	2-4	RIA solid phase method(IRMA) Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.	See below.
IGF-1 (Somatomedin C)	serum 0.5	S09 ↓ A00		（21 days）	2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	See below.
Prolactin	serum 0.4	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	Male 4.29-13.69 Premenopausal women 4.91-29.32 Postmenopausal women 3.12-15.39 (ng/mL)
Adrenocorticotropic hormone (ACTH)	plasma 0.5	PN2，PN5 ↓ A00		（21 days）	2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	at 7:00-10:00 7.2-63.3 (pg/mL)
Luteinizing hormone (LH)	serum 0.5	S09 ↓ A00			2-4	CLIA Chemiluminescent immunoassay (CLIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with chemiluminescentlabeled antibodies. The emission intensity of chemiluminescent substance is measured.	See below.
Follicle stimulating hormone (FSH)	serum 0.5	S09 ↓ A00			2-4	CLIA Chemiluminescent immunoassay (CLIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with chemiluminescentlabeled antibodies. The emission intensity of chemiluminescent substance is measured.	See below.
Thyroid stimulating hormone (TSH)	serum 0.5	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	0.500-5.00 (μIU/mL)
Antidiuretic hormone (AVP)	plasma 1.5	PN7 ↓ A00			5-7	RIA2 antibody assay Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	Water intake is restricted≦ 4.0Water intake is not restricted≦ 2.8(pg/mL)