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endocrinology test
gonadal hormones, placental hormones and their binding protein

Laboratory：Hachioji

○gonadal hormones, placental hormones and their binding protein

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Estradiol (E₂)	serum 0.5	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	See below.
IVF-Estradiol (E₂)	serum 0.5	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	(pg/mL)
Progesterone	serum 0.5	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	See below.
IVF-Progesterone	serum 0.5	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	(ng/mL)
Pregnanediol	urine collected for 24 hrs 12	U20		（28 days）	6-7	Gas chromatography-mass spectrometry(GC/MS),(enzymatic hydrolysis)	See below.
Pregnanetriol	urine collected for 24 hrs 12	U20		（28 days）	6-7	Gas chromatography-mass spectrometry(GC/MS),(enzymatic hydrolysis)	See below.
Testosterone	serum 0.4	S09 ↓ A00			2-4	ECLIA Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.	Male: 1.31-8.71 Female: 0.11-0.47 (ng/mL)
Testosterone, free RIA	serum 0.3	S09 ↓ A00			2-6	RIA solid phase method Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.	See below.
Human chorionic gonadotropin (HCG)	serum 0.5	S09 ↓ A00			2-4	CLEIA Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.	≦ 2.7 (mIU/mL)
Free HCG-β (HCG-β subunit)	serum 0.3	S09 ↓ A00			2-4	RIA solid phase method Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.	≦ 0.1 (ng/mL)
Free HCG-β (HCG-β subunit)	random urine 0.3	U00			2-4	RIA solid phase method Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.	≦ 0.1 (ng/mL)
Estradiol (E₂)（Suspended beyond orders placed 11-27-2019）	urine collected for 24 hrs 3	U00			8-14	RIA ammonium sulfate precipitation method Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	See below.
5α-dihydrotestosterone（Suspended beyond orders placed 12-05-2019）	serum 1.2	S09 ↓ A00			8-14	RIA ammonium sulfate precipitation method Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	Male: 0.20-1.00 Female: 0.05-0.30 (ng/mL)
Estrogen 3 fractionation〔 Estrone Estradiol Estriol〕（Suspended beyond orders placed 11-27-2019）	urine collected for 24 hrs 5	U00			8-14	RIA ammonium sulfate precipitation method Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	See below.
Estrogen, total Nonpregnant woman（Suspended beyond orders placed 12-05-2019）	urine collected for 24 hrs 5	U00			7-18	RIA ammonium sulfate precipitation method Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	See below.
Estrone (E₁)（Suspended beyond orders placed 11-27-2019）	urine collected for 24 hrs 5	U00			8-14	RIA ammonium sulfate precipitation method Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	See below.
Estriol (E₃)（Suspended beyond orders placed 11-27-2019）	urine collected for 24 hrs 5	U00			8-14	RIA ammonium sulfate precipitation method Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. 　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.	See below.