gonadal hormones, placental hormones and their binding protein
Laboratory:Hachioji
○gonadal hormones, placental hormones and their binding protein
TEST NAME
SPECIMEN REQUIREMENT (mL)
CONTAINER
CAP COLOR
STORE TEMPERATURE (STABILITY)
TURNAROUND TIME (DAY)
METHODOLOGY
REFERENCE RANGE (UNIT)
Estradiol (E2)
serum
0.5
S09 ↓ A00
2-4
ECLIA
Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.
See below.
IVF-Estradiol (E2)
serum
0.5
S09 ↓ A00
2-4
ECLIA
Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.
(pg/mL)
Progesterone
serum
0.5
S09 ↓ A00
2-4
ECLIA
Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.
See below.
IVF-Progesterone
serum
0.5
S09 ↓ A00
2-4
ECLIA
Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.
(ng/mL)
Pregnanediol
urine collected for 24 hrs
12
U20
(28 days)
6-7
Gas chromatography-mass spectrometry(GC/MS),(enzymatic hydrolysis)
See below.
Pregnanetriol
urine collected for 24 hrs
12
U20
(28 days)
6-7
Gas chromatography-mass spectrometry(GC/MS),(enzymatic hydrolysis)
See below.
Testosterone
serum
0.4
S09 ↓ A00
2-4
ECLIA
Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.
Male: 1.31-8.71 Female: 0.11-0.47 (ng/mL)
Testosterone, free RIA
serum
0.3
S09 ↓ A00
2-6
RIA solid phase method
Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.
See below.
Human chorionic gonadotropin (HCG)
serum
0.5
S09 ↓ A00
2-4
CLEIA
Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.
Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.
Immunoradiometric assay (IRMA) IRMA is one of the techniques of RIA. Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with antibodies labeled with radioactive isotope (RI). This is also called as the sandwich technique, because the solid-phase linked antibodies and the RI-labeled antibodies bind to the antigens, respectively, forming an antigen sandwich.
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
See below.
Estrogen, total Nonpregnant woman(Suspended beyond orders placed 12-05-2019)
urine collected for 24 hrs
5
U00
7-18
RIA ammonium sulfate precipitation method
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
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