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serum immunological test
immunoglobulin

Laboratory：Hachioji

○immunoglobulin

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
IgG	serum 0.4	S09 ↓ A00			2-4	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	870-1700 (mg/dL)
IgA	serum 0.4	S09 ↓ A00			2-4	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	110-410 (mg/dL)
IgM	serum 0.4	S09 ↓ A00			2-4	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	Male: 33-190 Female: 46-260 (mg/dL)
IgG, subclass fractionation (TIA)	serum 0.5	S09 ↓ A00			2-6	Turbidimetric immunoassay and latex turbidimetric immunoassay	IgG1: 351-962 IgG2: 239-838 IgG3: 8.5-140 IgG4: 4.5-117 (mg/dL)
IgG2	serum 0.5	S09 ↓ A00		（28 days）	2-6	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	239-838 (mg/dL)
Immunoelectrophoresis, identification by anti-total human serum	serum 0.2	S09 ↓ A00			4-6	Immunoelectrophoresis(IEP) Electrophoresis A technique for analysis of macromolecules based on the following phenomena: When an electric current is applied to an electrolyte solution in which charged particles suspended, the particles migrate towards the electrode of opposite charge. Based on the length of migration, the target molecule is determined. Cellulose acetate membrane, agarose gel, and polyacrylamide gel are used as support media.
Immunoelectrophoresis, specific antiserum	serum 0.4	S09 ↓ A00			4-6	Immunofixation
Immunoglobulin free light chain κ/λ	serum 0.8	S09 ↓ A00			2-4	Turbidimetric immunoassay(TIA) Turbidimetric immunoassay (TIA) A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.	κ: 3.3-19.4 (mg/L) λ: 5.7-26.3 (mg/L) κ/λ ratio: 0.26- 1.65
Immunoelectrophoresis, specific antiserum (DIRA)	serum 0.4	S09 ↓ A00			4-8	Immunofixation
Urine immunoelectrophoresis, Bence Jones protein identification	random urine 0.4	U00			3-5	Immunoelectrophoresis(IEP)
Oligoclonal band	serum and CSF 1.0 and 1.0	S09 ↓ A00 and A00			10-14	Isoelectric focusing Electrophoresis A technique for analysis of macromolecules based on the following phenomena: When an electric current is applied to an electrolyte solution in which charged particles suspended, the particles migrate towards the electrode of opposite charge. Based on the length of migration, the target molecule is determined. Cellulose acetate membrane, agarose gel, and polyacrylamide gel are used as support media.	None-detected
Cryoglobulin, qualitative	serum 1.0	S09 ↓ A00			4-6	Cold precipitation	Negative
IgG4	serum 0.5	S09 ↓ A00		（28 days）	2-4	Latex turbidimetric immunoassay	11-121 (mg/dL)