genetic tests for hematopoietic tumor

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genetic tests for hematopoietic tumor

Laboratory：Hachioji

○genetic tests for hematopoietic tumor

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
FLT3/ITD mutation analysis	bone marrow fluid 1.0	H00			7-11	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.	No detection of ITD mutation
FLT3/ITD mutation analysis	whole blood (with EDTA-2Na) 2.0	PN2，PN5			7-11	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.	No detection of ITD mutation
FLT3 mutational analysis ITD/TKD	bone marrow fluid 1.0	H00		（7 days）	4-8	PCR-Capillary electrophoresis method	Negative
FLT3 mutational analysis ITD/TKD	whole blood (with heparin) 3.0	PH5		（7 days）	4-8	PCR-Capillary electrophoresis method	Negative
NPM1 mutational analysis	bone marrow fluid 1.0	H00			10-16	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the mutation
NPM1 mutational analysis	whole blood (with EDTA-2Na) 2.0	PN2，PN5			10-16	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the mutation
KIT sequence analysis (leukemia)	bone marrow fluid 1.0	H00			10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.	No detection of the mutation
KIT sequence analysis (leukemia)	whole blood (with EDTA-2Na) 7.0	PN7			10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.	No detection of the mutation
JAK2V617F mutational analysis	whole blood (with EDTA-2Na) 5.0	PN5		（3 days）	4-8	Allelespecific quantitative PCR (Realtime PCR)	≤ １.000% Negative
Screening of chimeric genes related to leukemia,quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	(copy/μg RNA)
MPN gene mutational analysis	whole blood (with EDTA-2Na) 5.0	PN5		（3 days）	4-8	PCR	Mutation not detected.
Screening of chimeric genes related to leukemia,quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	(copy/μg RNA)
WT1 mRNA quantitative	whole blood (with EDTA-2Na) 7.0	PN7			4-6	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	(copy/μg RNA)
WT1 mRNA quantitative	bone marrow fluid 1.0	H00			4-6	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	(copy/μg RNA)
Major BCR-ABL1 mRNA, quantitative	bone marrow fluid 1.0	H00		（1 day）	3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detectionof thechimericmRNA（copy/μgRNA）
Major BCR-ABL1 mRNA (IS)	whole blood (with EDTA-2Na) 7.0	PN7		（1 day）	4-6	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.
Major BCR-ABL1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
Major BCR-ABL1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
Mutation analysis in the ABL1 region, major BCR-ABL1	bone marrow fluid 1.0	H00		（1 day）	10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.
Mutation analysis in the ABL1 region, major BCR-ABL1	whole blood (with EDTA-2Na) 7.0	PN7		（1 day）	10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.
minor BCR-ABL1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
minor BCR-ABL1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
minor BCR-ABL1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
minor BCR-ABL1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
Mutation analysis in the ABL1 region, minor BCR-ABL1	bone marrow fluid 1.0	H00		（1 day）	10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.
Mutation analysis in the ABL1 region, minor BCR-ABL1	whole blood (with EDTA-2Na) 7.0	PN7		（1 day）	10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.
TCF3-PBX1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
TCF3-PBX1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
TCF3-PBX1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
TCF3-PBX1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
PML-RARA mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
PML-RARA mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
PML-RARA mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
PML-RARA mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
CBFB-MYH11 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
CBFB-MYH11 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
CBFB-MYH11 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
CBFB-MYH11 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
RUNX1-RUNX1T1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
RUNX1-RUNX1T1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
RUNX1-RUNX1T1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
RUNX1-RUNX1T1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
RUNX1-MECOM mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
RUNX1-MECOM mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
ETV6-RUNX1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
ETV6-RUNX1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
ETV6-RUNX1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
ETV6-RUNX1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-AFF1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-AFF1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-AFF1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-AFF1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-AFDN mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-AFDN mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-AFDN mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-AFDN mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-MLLT3 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-MLLT3 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-MLLT3 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-MLLT3 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-MLLT1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-MLLT1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
KMT2A-MLLT1 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
KMT2A-MLLT1 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
NUP98-HOXA9 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
NUP98-HOXA9 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
STIL-TAL1 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
STIL-TAL1 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
DEK-NUP214 mRNA, quantitative	bone marrow fluid 1.0	H00			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
DEK-NUP214 mRNA, quantitative	whole blood (with EDTA-2Na) 7.0	PN7			3-5	RT-PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	No detection of the chimeric mRNA (copy/μg RNA)
DEK-NUP214 mRNA, qualitative	bone marrow fluid 1.0	H00			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
DEK-NUP214 mRNA, qualitative	whole blood (with EDTA-2Na) 7.0	PN7			6-9	RT-PCR Reverse transcriptase-polymerase chain reaction (RT-PCR) When RNA to be amplified by PCR, complementary DNA (cDNA) is synthesized using reverse transcriptase (RT) using RNA as a template.	No detection of the chimeric mRNA
JAK2 V617F mutation analysis（Suspended beyond orders placed 01-31-2020）	whole blood (with EDTA-2Na) 5.0	PN5			8-12	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	Mutation not detected
MPL/CALR mutation analysis（Suspended beyond orders placed 03-31-2020）	whole blood (with EDTA-2Na) 5.0	PN5			4-8	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.	Mutation not detected
JAK2 V617F mutation analysis（Suspended beyond orders placed 01-31-2020）	bone marrow fluid 1.0	H00			8-12	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	Mutation not detected

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