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viral infection test

Laboratory：Hachioji

○viral infection test

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Varicella-zoster virus DNA quantitative	whole blood (with EDTA-2Na) 5.0	PN7			2-4	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	< 2.0×101 (copy/106 cells)
Human herpesvirus, type 6 DNA quantitative	whole blood (with EDTA-2Na) 5.0	PN7			2-4	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.	< 2.0×101 (copy/106 cells)
HCV-1b-IFN/ ribavirin mutation	serum 0.5	S3F,S5F			10-14	Direct sequencing method Direct sequencing method Direct sequence analysis using PCR-amplified DNA as a sequencing template.	AA70: None- detectedAA91: None- detected
Measles virus antibodies for vaccination (particle agglutination assay, PA)（Suspended beyond orders placed 12-01-2022）	serum 0.3	S09 ↓ A00			3-5	Particle agglutination(PA) Particle agglutination (PA) Using antigen- or antibody-coated gelatin particles (sensitizing particles), etc., the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots.	(x)