lipids and related materials

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lipids and related materials

Laboratory：Hachioji

○lipids and related materials

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Triglycerides (TG)	Serum 0.5	S09 ↓ A00		（1 month）	2-4	Enzymatic method(GK-GPO free glycerol blank) Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	50～149(mg/dL)
Phospholipid	Serum 0.5	S09 ↓ A00		（21 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	160～260(mg/dL)
Free (or non-esterified) fatty acid(NEFA)	Serum 0.3	S09 ↓ A00		（28 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	140～850(μEq/L)
Total cholesterol (T-Cho)	Serum 0.5	S09 ↓ A00		（1 month）	2-4	Cholesterol oxidase (COD-POD) method	150～219(mg/dL)
Esterified cholesterol (Echo)	Serum 0.5	S09 ↓ A00		（28 days）	2-4	Cholesterol oxidase(COD-POD) method, Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	90～200(mg/dL)
Free cholesterol (F-Cho)	Serum 0.5	S09 ↓ A00		（28 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	30～60(mg/dL)
Cholesterol ester rate	Serum 0.5	S09 ↓ A00		（28 days）	2-4	Cholesterol oxidase(COD-POD) method, Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	73～77(%)
HDL-cholesterol	Serum 0.5	S09 ↓ A00		（1 month）	2-4	direct method	M40~86F40~96(mg/dL)
HDL_{2, 3}-cholesterol	Serum 1	S09 ↓ A00		（20 days）	2-6	ultracentrifugation method Ultracentrifugation method A method of separating and measuring proteins based on the difference in their specific gravity using an ultracentrifuge.	HDL2 Cholesterol M 16.0-61.0 F 23.0-73.0 HDL3 Cholesterol M 13.0-25.0 F 13.0-24.0 (mg/dL)
LDL-cholesterol	Serum 0.5	S09 ↓ A00		（1 month）	2-4	direct method	70~139(mg/dL)
Remnant-like particles-cholesterol (RLP-C)	Serum 0.5	S09 ↓ A00			2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	7.5 or less (mg/dL)
sd LDL-cholesterol	Serum 0.5	S09 ↓ A00		（5 days）	2-4	direct method	Less than 35.0 (mg/dL)
Oxidized LDL (MDA-LDL)	Serum 0.3	S09 ↓ A00		（21 days）	3-14	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	Male Under 45 years old 46-82 Over 45 years old 61-105 Female Under 55 years old 46-82 Over 55 years old 61-105 (U/L)
sd LDL-C (physical examination)	Serum 0.5	S09 ↓ A00		（5 days）	2-4	direct method	(mg/dL)
Fatty acid fractionation (24)	Plasma 0.5	PH5 ↓ A00		（45 days）	10-14	Gas-Chromatograph method Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	See below
Fatty acid fractionation (24)	Serum 0.5	S09 ↓ A00		（45 days）	10-14	Gas-Chromatograph method Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	See below
Fatty acid fractionation (4)	Plasma 0.5	PH5 ↓ A00		（45 days）	4-6	Gas-Chromatograph method Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61 DHA/AA ratio 0.27-1.07 (EPA +DHA)/ AA ratio 0.32-1.66
Fatty acid fractionation (4)	Serum 0.5	S09 ↓ A00		（45 days）	4-6	Gas-Chromatograph method Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61 DHA/AA ratio 0.27-1.07 (EPA +DHA)/ AA ratio 0.32-1.66
Very long chain fatty acid	Plasma 2	PH5 ↓ A00		（1 month）	6-19	Gas chromatography method (GC-MS) Chromatography A substance is passed between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) flowing in contact with it, and the difference in affinity for both phases is used to generate the target substance. How to separate components. When the mobile phase is liquid, it is called liquid chromatography, and when it is gas, it is called gas chromatography.	C24:0/C22:00.628～0.977C25:0/C22:00.012～0.023C26:0/C22:00.003～0.006
Total bile acids	Serum 0.5	S09 ↓ A00			2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	10.0 or less (μmol/L)
β-lipoprotein	Serum 0.5	S09 ↓ A00		（21 days）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	220～650(mg/dL)
Lipoprotein fractionation	Serum 0.2	S09 ↓ A00		（7 days）	2-4	Agarose gel electrophoresis Electrophoresis method A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.	α M26.9～50.5 F32.6～52.5 PREβ M7.9～23.8 F6.6～20.8 β M35.3～55.5 F33.6～52.0 (%)
Cholesterol fractionation	Serum 0.2	S09 ↓ A00		（7 days）	2-4	Agarose gel electrophoresis Electrophoresis method A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.	HDL cholesterol 23-48 LDL cholesterol 47-69 VLDL cholesterol 2-15 (%)
Lipoprotein (a)	Serum 0.5	S09 ↓ A00		（1 month）	2-4	LA (latex agglutination turbidimetry) LA (Latex agglutination immunoassay) Latex agglutination turbidimetry An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.	40 or less (Reference value)(mg/dL)
Lipoprotein lipase (LPL)	Plasma 0.3	PH5 ↓ A00		（1 month）	2-8	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	164～284(ng/mL)
Apolipoprotein A-I	Serum 0.3	S09 ↓ A00		（1 month）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	M119～155F126～165(mg/dL)
Apolipoprotein A-II	Serum 0.3	S09 ↓ A00		（1 month）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	M25.9～35.7F24.6～33.3(mg/dL)
Apolipoprotein B	Serum 0.3	S09 ↓ A00		（1 month）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	M73～109F66～101(mg/dL)
Apolipoprotein C-II	Serum 0.3	S09 ↓ A00		（1 month）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	M1.8～4.6F1.5～3.8(mg/dL)
Apolipoprotein C-III	Serum 0.3	S09 ↓ A00		（1 month）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	M5.8～10.0F5.4～9.0(mg/dL)
Apolipoprotein E	Serum 0.3	S09 ↓ A00		（1 month）	2-4	immunoturbidimetric method TIA (Turbidimetric immnoassay) Immunoturbidimetry A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.	M2.7～4.3F2.8～4.6(mg/dL)
Apolipoprotein E phenotype	Serum 0.2	S09 ↓ A00		（7 days）	4-20	Immunoblot method using isoelectric focusing method Electrophoresis method A method that uses the phenomenon that when electricity is applied to an electrolyte solution in which charged particles are suspended, the particles move toward the polarity opposite to the charge on each particle, and the target substance is measured from its mobility. Cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used as the aqueous support.
Pulmonary surfactant protein A (SP-A)	Serum 0.3	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 43.8 (ng/mL)
Pulmonary surfactant protein D (SP-D)	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Less than 110(ng/mL)
Lipoprotein Cholesterol (LP-C) Fractional Quantitation（Suspended beyond orders placed 12-03-2020）	Serum 1.0	S09 ↓ A00		（20 days）	2-4	ultracentrifugation method Ultracentrifugal method A method of separating and measuring proteins by the difference in specific gravity using an ultracentrifuge.	See below
Glycolic acid(CG)（Suspended beyond orders placed 03-31-2022）	Serum 0.2	S09 ↓ A00			2-8	RIA PEG method RIA (Radio immunoassay) Radioimmunoassay A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity. As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.	50 or less (μg/dL)
Fatty acid fraction of total lipids（Suspended beyond orders placed 12-29-2017）	Serum 0.5	S09 ↓ A00		（1 month）	10-14	Gas-Chromatograph method Chromatography A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases. It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.	See below
Fatty acid fraction of total lipids（Suspended beyond orders placed 12-29-2017）	Plasma 0.5	PH5 ↓ A00		（1 month）	10-14	Gas-Chromatograph method Chromatography A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases. It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.	See below
Blood fatty acid fraction（Suspended beyond orders placed 12-29-2017）	Plasma 0.5	PH5 ↓ A00		（35 days）	4-6	Gas-Chromatograph method Chromatography A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases. It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.	Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61
Blood fatty acid fraction（Suspended beyond orders placed 12-29-2017）	Serum 0.5	S09 ↓ A00		（35 days）	4-6	Gas-Chromatograph method Chromatography A method for passing a substance between a stationary phase (solid or liquid) and a mobile phase (liquid or gas) that flows in contact with it, and separating the components of the target substance using the difference in affinity for the two phases. It is called liquid chromatography when the mobile phase is liquid, and gas chromatography when the mobile phase is gas.	Dihomo-γ-linolenic acid 22.6-72.5 Arachidonic acid 135.7-335.3 Eicosapentaenoic acid 10.2-142.3 Docosahexaenoic acid 54.8-240.3 (μg/mL) EPA/AA ratio 0.05-0.61
Free fatty acids(FFA，NEFA)（Suspended beyond orders placed 06-30-2016）	Serum 0.3	S09 ↓ A00		（1 month）	2-4	Enzymatic method Enzyme method The measurement principle is the same as the colorimetric method, and the measurement substance is specifically measured using an enzyme.	140-850(μEq/L)
L-CAT（Suspended beyond orders placed 03-31-2016）	Serum 1.5	S09 ↓ A00				Dipalmitoyl lecithin substrate method
Lipoprotein lipid fraction〔 Lipoprotein cholesterol fraction quantitation Lipoprotein trigLipoprotein triglyceride fraction determination Lipoprotein phospholipid fraction determination〕（Suspended beyond orders placed 12-03-2020）	Serum 1.0	S09 ↓ A00			2-4	ultracentrifugation method Ultracentrifugal method A method of separating and measuring proteins by the difference in specific gravity using an ultracentrifuge.	See below
Lipoprotein fraction（Suspended beyond orders placed 09-30-2021）	Serum 0.5	S09 ↓ A00		（7 days）	3-7	Polyacrylamide gel disk electrophoresis method Electrophoresis A method using the phenomenon that, when electricity is applied to an electrolyte solution with suspended charged particles, the particles migrate toward the polarity opposite to the charge on each particle, to measure the analyte based on its mobility. For the aqueous solution support, cellulose acetate membrane, agarose gel, polyacrylamide gel, etc. are used.	HDL M22-47 F27-50 LDL M46-68 F44-66 VLDL M3-19 F2-12 (%)

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