TEST DIRECTORY

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Laboratory：Hachioji

○infections disease (non virus) related test

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

Chlamydia trachomatis IgA

serum
0.2
S09
↓
A00 		Refrigeration
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: < 0.90
See the following criteria.

Chlamydia trachomatis IgG

serum
0.2
S09
↓
A00 		Refrigeration
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: < 0.90
See the following criteria.

Chlamydia trachomatis DNA

secretion
V50 Room temperature
（28 days）
 2-4 PCR(Real-time PCR)

Real-time PCR
Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.

 Negative

Chlamydia trachomatis DNA

random urine
5
U10 Room temperature
（28 days）
 2-4 PCR(Real-time PCR)

Real-time PCR
Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.

 Negative

Chlamydia trachomatis DNA

mouth washing liquid
5
U10 Room temperature
（28 days）
 2-4 PCR(Real-time PCR)

Real-time PCR
Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.

 Negative

Chlamydophila psittaci (Chlamydia psittaci)

Immediately
serum
0.3
S09
↓
A00 		Refrigeration
 4-6 CF

Complement fixation (CF)
A technique based on the principle that complements bind with antigen-antibody complexes and that they mediate hemolysis.
When red blood cells coated with hemolysin (sensitized red blood cells) bind to complements, hemolysis occurs; however, in the existence of the antigen-antibody complexes, complements are used, resulting in no hemolysis. Based on the hemolytic reaction, the presence of a specific antibody is determined.

 < 4
(x)

Chlamydophila (Chlamydia) psittaci IgG

serum
0.2
S09
↓
A00 		Refrigeration
 3-9 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Chlamydophila (Chlamydia) psittaci IgM

serum
0.2
S09
↓
A00 		Refrigeration
 3-9 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Chlamydophila (Chlamydia) pneumoniae IgG

serum
0.2
S09
↓
A00 		Refrigeration
（21 days）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: < 30
See the following criteria.

Chlamydophila (Chlamydia) pneumoniae IgA

serum
0.2
S09
↓
A00 		Refrigeration
（21 days）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: < 8
See the following criteria.

Chlamydophila (Chlamydia) pneumoniae IgM

serum
0.2
S09
↓
A00 		Refrigeration
（21 days）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: < 0.5
See the following criteria.

Antistreptolysin-O antibody (ASO)

serum
0.6
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay ≦ 239
(IU/mL)

Endotoxin, quantitative

whole blood (with Novoheparin)
2.0
PE2 Refrigeration
 2-4 Turbiditytime analysis ≦ 1.0
(cutoff)
(pg/mL)

Endotoxin in dialysis fluid Solution A

dialysis fluid
4.0
PE4 Refrigeration
 2-4 Chromogenic substrate method

Chromogenic substrate method
By adding heparin, an antithrombin III (AT-III)-heparin complex is generated. Then, the thrombin inactivation capacity is measured using a chromogenic substrate for thrombin.

 (EU/mL)

Endotoxin in dialysis fluid Solution B

dialysis fluid
4.0
PE4 Refrigeration
 2-4 Chromogenic substrate method

Chromogenic substrate method
By adding heparin, an antithrombin III (AT-III)-heparin complex is generated. Then, the thrombin inactivation capacity is measured using a chromogenic substrate for thrombin.

 (EU/mL)

Endotoxin in dialysis fluid RO water

dialysis fluid
4.0
PE4 Refrigeration
 2-4 Chromogenic substrate method

Chromogenic substrate method
By adding heparin, an antithrombin III (AT-III)-heparin complex is generated. Then, the thrombin inactivation capacity is measured using a chromogenic substrate for thrombin.

 (EU/mL)

Endotoxin in dialysis fluid Preparation solution

dialysis fluid
4.0
PE4 Refrigeration
 2-4 Chromogenic substrate method

Chromogenic substrate method
By adding heparin, an antithrombin III (AT-III)-heparin complex is generated. Then, the thrombin inactivation capacity is measured using a chromogenic substrate for thrombin.

 (EU/mL)

Endotoxin in dialysis fluid Others

dialysis fluid
4.0
PE4 Refrigeration
 2-4 Chromogenic substrate method

Chromogenic substrate method
By adding heparin, an antithrombin III (AT-III)-heparin complex is generated. Then, the thrombin inactivation capacity is measured using a chromogenic substrate for thrombin.

 (EU/mL)

Bordetella antibody, EIA

serum
0.2
S09
↓
A00 		Refrigeration
（28 days）
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 PT-IgG: < 10
FHA-IgG: < 10
(EU/mL)

Bordetella antibody, IgA

serum
0.2
S09
↓
A00 		Refrigeration
（28 days）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: <8.5
See the following criteria.

Bordetella antibody, IgM

serum
0.2
S09
↓
A00 		Refrigeration
（28 days）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: <8.5
See the following criteria.

Anti-helicobacter pylori antibody, LA

serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Latex agglutination immunoassay < 10
(U/mL)

Fecal Helicobacter pylori antigen

stool
(1 dedicated stick)
F20 Refrigeration
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative

Syphilis serology test, qualitative, RPR, LA

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex turbidimetry Negative

Syphilis serology test, quantitative RPR, LA

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex turbidimetry < 1.0
(R.U.)

Syphilis serology test, quantitative RPR

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 Agglutination Negative (< 1)
(x)

Treponema pallidum, qualitative, TP antibody, LA

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex turbidimetry Negative

Treponema pallidum, quantitative TP antibody, LA

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex turbidimetry < 5
(U/mL)
See the
following
criteria.

Treponema pallidum, quantitative TPHA

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 Particle agglutination(PA)

Particle agglutination (PA)
Using antigen- or antibody-coated gelatin particles (sensitizing particles), etc., the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots.

 Negative < 1:80
(x)

FTA-ABS

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 Negative

Cold hemagglutinins

Immediately
serum
0.3
S09
↓
A00 		Refrigeration
 3-5 Hemagglutination(HA)

Hemagglutination (HA)
Red blood cell surface antigens are reacted with the antibodies. After the antigen-antibody reaction is completed, the presence of the antibody is determined based on appearance or non-appearance of clots.

 < 256
(x)

Mycoplasma pneumoniae

Immediately
serum
0.3
S09
↓
A00 		Refrigeration
 4-6 CF

Complement fixation (CF)
A technique based on the principle that complements bind with antigen-antibody complexes and that they mediate hemolysis.
When red blood cells coated with hemolysin (sensitized red blood cells) bind to complements, hemolysis occurs; however, in the existence of the antigen-antibody complexes, complements are used, resulting in no hemolysis. Based on the hemolytic reaction, the presence of a specific antibody is determined.

 < 4
(x)

Mycoplasma pneumoniae

serum
0.2
S09
↓
A00 		Refrigeration
 2-4 Particle agglutination(PA)

Particle agglutination (PA)
Using antigen- or antibody-coated gelatin particles (sensitizing particles), etc., the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots.

 < 40
(x)

Rickettsia tsutsugamushi, Karp, IgG

serum
0.2
S09
↓
A00 		Refrigeration
 3-6 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Rickettsia tsutsugamushi, Karp, IgM

serum
0.2
S09
↓
A00 		Refrigeration
 3-6 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Rickettsia tsutsugamushi, Kato, IgG

serum
0.2
S09
↓
A00 		Refrigeration
 3-6 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Rickettsia tsutsugamushi, Kato, IgG

serum
0.2
S09
↓
A00 		Refrigeration
 3-6 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Rickettsia tsutsugamushi, Gilliam, IgG

serum
0.2
S09
↓
A00 		Refrigeration
 3-6 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Rickettsia tsutsugamushi, Gilliam, IgM

serum
0.2
S09
↓
A00 		Refrigeration
 3-6 FA

Fluorescent antibody method (FA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then fluorochrome-labeled antibodies for the secondary reaction.

 < 10
(x)

Cryptococcus neoformans antigen

serum
0.8
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination

Latex agglutination (LA)
Using antigen-or antibody-coated latex particles (sensitizing latex particles), the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots

 Negative

Cryptococcus neoformans antigen

CSF
0.8
A00 Refrigeration
 2-4 Latex agglutination

Latex agglutination (LA)
Using antigen-or antibody-coated latex particles (sensitizing latex particles), the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots

 Negative

recommission
Cryptococcus antibody

serum
1.0
S7P
↓
A00 		Freeze
 14-16 Tube agglutination method < 2
(x)

Candida mannan antigen

serum
0.8
S09
↓
A00 		Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 < 0.05
(U/mL)

recommission
Candida antibody

serum
1.0
S7P
↓
A00 		Freeze
 14-16 Immunodiffusion (-)

INACTIVE
Aspergillus antibody（Suspended beyond orders placed 10-31-2022）

serum
1.0
S09
↓
A00 		Refrigeration
 5-7 Ouchterlony

Ouchterlony method(Ouchterlony double immunodiffusion)
One of the gel-diffusion techniques, also called as ouchterlony double immunodiffusion, to identify antigen and antibody reactions. After antibodies and antigens are diffused in the gel plate, the presence or absence and the number of precipitin lines formed by the antigen-antibody reaction are confirmed.

 Negative

Aspergillus antigen

serum
1.0
S09
↓
A00 		Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative < 0.5

β-D-glucan

whole blood (with Novoheparin)
2.0
PE2 Refrigeration
 2-4 Chromogenic substrate method

Chromogenic substrate method
By adding heparin, an antithrombin III (AT-III)-heparin complex is generated. Then, the thrombin inactivation capacity is measured using a chromogenic substrate for thrombin.

 ≦ 20.0
(cutoff )
(pg/mL)

Aspergillus antibody IgG

serum
0.3
S09
↓
A00 		Refrigeration
（28 days）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 ＜5.0
Negative
(AU/mL)
Criteria ： See below.

Anti-Trichosporon asahii antibody

serum
0.3
S09
↓
A00 		Freeze
（28 days）
 7-14 Antigen-captured ELISA < 0.15
(CAI)
See the following criteria.

Toxoplasma antibody, IgG

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 < 6
See the following criteria.

Toxoplasma antibody, IgM

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 < 0.8
See the following criteria.

INACTIVE
Anti-anisakis antibody IgG-A（Suspended beyond orders placed 03-26-2021）

serum
0.3
S09
↓
A00 		Freeze
 9-14 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 ≦ 1.50
(INDEX)

Tuberculosis specific IFN-γ

whole blood (with heparin)
5.0
PH5 Room temperature
（54 hours）
 3-5 ELISPOT

Enzyme-linked immunospot (ELISPOT)
In a plate coated with a specific antibody, isolated cells and a specific antigen are added and cultured. Proteins secreted by the cells are reacted with a chromogenic
substrate to generate spots.

 Negative

Bird allergenspecific IgG antibody

serum
0.5
S09
↓
A00 		Refrigeration
（7 days）
 3-9 FEIA

Fluorescence enzyme immunoassay (FEIA)
One of the techniques of EIA. Using either enzyme-labeled antigen or antibody as a labeled-substance, the antigenantibody reaction is carried out. After adding a fluorescence substrate, the fluorescence intensity is measured.

 Negative
Criteria：See below.
(mgA/L)

Bird allergenspecific IgG antibody

plasma
0.5
PN2,PN5
↓
A00 		Refrigeration
（7 days）
 3-9 FEIA

Fluorescence enzyme immunoassay (FEIA)
One of the techniques of EIA. Using either enzyme-labeled antigen or antibody as a labeled-substance, the antigenantibody reaction is carried out. After adding a fluorescence substrate, the fluorescence intensity is measured.

 Negative
Criteria：See below.
(mgA/L)

Anti-GPL core of Mycobacterium avium complex antibody (Anti- Mycobacterium antibody, qualitative)

serum
0.5
S09
↓
A00 		Refrigeration
（15 days）
 3-5 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative
＜0.7
(U/mL)

INACTIVE recommission
Cryptococcus antibody（Suspended beyond orders placed 09-10-2022）

CSF
1.0
A00 Freeze
 14-16 IFA

Indirect fluorescent antibody method (IFA)
Using fluorochrome-labeled antibodies to the target antigen, the antigen-antibody reaction is carried out, and the fluorescence intensity is measured using fluorescence microscope.
The direct method uses fluorochrome-labeled antibodies that react directly with the antigen. The indirect method uses antibodies that react with the antigen first and then
fluorochrome-labeled antibodies for the secondary reaction.

 < 1
(x)

INACTIVE
Antistreptokinase antibody (ASK)（Suspended beyond orders placed 12-01-2022）

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 Particle agglutination(PA)

Particle agglutination (PA)
Using antigen- or antibody-coated gelatin particles (sensitizing particles), etc., the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots.

 Adult: < 1:2560
Child: Negative
< 1:5120
(x)

INACTIVE
Candida antigen（Suspended beyond orders placed 05-29-2020）

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination

Latex agglutination (LA)
Using antigen-or antibody-coated latex particles (sensitizing latex particles), the antigen-antibody reaction is carried out. The existence of the antibody or antigen is determined based on appearance or non-appearance of clots

 Negative (< 2)
(x)

INACTIVE
Chlamydia trachomatis Immunoglobulin M (IgM)（Suspended beyond orders placed 12-05-2019）

serum
0.2
S09
↓
A00 		Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative: < 0.90
See the
following
criteria.

INACTIVE
Anti-helicobacter pylori antibody, IgG（Suspended beyond orders placed 03-31-2020）

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 < 10
(U/mL)

INACTIVE
Brucella agglutination（Suspended beyond orders placed 10-22-2020）

serum
1.0
S09
↓
A00 		Freeze
 Please contact us in advance. Bacteria agglutination Abortus: < 1:40
Canis: < 1:160
(x)

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