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microbiological test
Acid-fast bacteria

Laboratory：Hachioji

○Acid-fast bacteria

TEST NAME	TESTING MATERIAL	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	METHODOLOGY	TURNAROUND TIME (DAY)
Acid-fast bacteria smear Concentrated fluorescence	Specimens such as sputum, bronchial lavage fluid, gastric juice, midstream urine, and pleural fluid	See below		See below	Concentrated fluorescence	2-4
Isolation and culture of acid-fast bacteria, liquid medium	Specimens such as sputum, bronchial lavage fluid, gastric juice, midstream urine, and pleural fluid	See below		See below	Oxygen-sensitive fluorescence sensor method	Up to 6weeks
Isolation and culture of acid-fast bacteria, Ogawa medium	Specimens such as sputum, bronchial lavage fluid, gastric juice, midstream urine, and pleural fluid	See below		See below	Modified Ogawa method	4-8weeks
Acid-fast bacteria identification (mass spectrometry)	Culture-positive strain or strain				MALDI-TOF-MS MALDI-TOF-MS An ionization technique that uses laser energy absorbing matrix to create ions by irradiating a laser to an analyte mixture without decomposing proteins is known as matrixassisted laser desorption/ionization (MALDI). MALDI is combined with a technique of time-of-flight mass spectrometry (TOF-MS) which is based on the principle that the ion’s time of flight changes depending on the formed ion’s mass-to-charge ratio. MALDI: Matrix-assisted laser desorption/ionization TOF-MS: Time-of-flight mass spectrometry	2-5
M. tuberculosis complex antigen	Culture-positive strain or strain				Immunochromatography	2-4
Drug susceptibility test for acid-fast bacteria	Culture-positive strain or strain				Single concentration ratio method	2-4weeks
Susceptibility test for Mycobacterium tuberculosis [MIC]	Culture-positive strain or strain				Broth microdilution method	2-3weeks
Susceptibility test for acid-fast bacteria [MIC]	Culture-positive strain or strain				Broth microdilution method	2-3weeks