cytokines | TEST DIRECTORY

Laboratory：Hachioji

○cytokines

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Soluble IL-2 receptor (sIL-2R)	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	157-474(U/mL)
TARC (Th2 chemokine)	Serum 0.3	S09 ↓ A00		（28 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	6 months to 12 months old: less than 1367 1 year to 2 years old: less than 998 2 years to 16 years old: less than 743 16 years and older: less than 450 (pg/mL)
TARC(COVID-19)	Serum 0.3	S09 ↓ A00		（28 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Cutoff value 95.1 or higher (pg/mL)
Interferon-lambda3 (IFN-λ3)	Serum 0.5	S09 ↓ A00		（22 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	Cutoff value less than 13.6 (pg/mL)
Human interferon γ〔EIA〕	Serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	Enzyme immunoassay(EIA) EIA (Enzyme immunoassay) Enzyme immunoassay The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.	0.1 or less (reference value) (IU/mL)
IL-1β (interleukin-1β)	Serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	10 or less (reference value) (pg/mL)
IL-4 (interleukin-4) (high sensitivity)	Serum 0.3	S09 ↓ A00		（1 month）	Please contact us in advance.	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	6.0 or less (Reference value)(pg/mL)
Interleukin-6 (IL-6)	Serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	5.8 or less (pg/mL)
IL-6 (interleukin-6)	Serum 0.5	S09 ↓ A00		（28 days）	2-5	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) Chemiluminescent enzyme immunoassay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.	4.0 or less (pg/mL)
IL-8 (interleukin-8)	Culture medium 0.5	A00			Please contact us in advance.	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	(pg/mL)
IL-8 (interleukin-8)	Plasma 0.5	PN2,PN5 ↓ A00			Please contact us in advance.	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	2.0 or less (Reference value)(pg/mL)
IL-10 (interleukin-10)	Serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	ELISA ELISA (Enzyme-Linked immunosorbent assay) Enzyme-linked immunosorbent assay After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.	5 or less (Reference value)(pg/mL)
Soluble ICAM-1（Suspended beyond orders placed 03-29-2019）	Serum 0.3	S09 ↓ A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	98.8-320(Reference value)(ng/mL)
IL-1α (interleukin-1α)（Suspended beyond orders placed 11-30-2017）	Culture medium 1.0	A00			Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	(pg/mL)
Soluble ELAM-1（Suspended beyond orders placed 03-29-2019）	Serum 0.3	S09 ↓ A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	17.9-79.2 (reference value) (ng/mL)
GM-CSF (Granulocyte/Macrophage Colony Stimulating Factor)（Suspended beyond orders placed 03-29-2019）	Culture medium 0.5	A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	(pg/mL)
Soluble VCAM-1（Suspended beyond orders placed 03-29-2019）	Serum 0.3	S09 ↓ A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	277-836(Reference value)(ng/mL)
IL-1RA (Interleukin-1 Receptor Antagonist)（Suspended beyond orders placed 11-30-2017）	Plasma 0.5	PN2,PN5 ↓ A00			Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	105-1062 (reference value) (pg/mL)
IL-3 (Interleukin-3)（Suspended beyond orders placed 11-30-2017）	Culture medium 1.0	A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	(pg/mL)
IL-1α (interleukin-1α)（Suspended beyond orders placed 11-30-2017）	Serum 1.0	S09 ↓ A00			Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	(pg/mL)
G-CSF (granulocyte colony-stimulating factor)（Suspended beyond orders placed 12-03-2020）	Serum 0.5	S09 ↓ A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	39.0 or less (Reference value)(pg/mL)
TGF-β1（Suspended beyond orders placed 12-03-2020）	Plasma 0.5	PN2,PN5 ↓ A00		（1 month）	Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	1.56-3.24 (reference value) (ng/mL)
Soluble IL-2 Receptor（Suspended beyond orders placed 03-29-2019）	Serum 0.5	S09 ↓ A00			2-4	CLEIA CLEIA (Chemiluminescent enzyme immunoassay) A method in which an antigen is reacted with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, a chemiluminescent substrate is added and the luminescence intensity is measured.	145-519(U/mL)
IL-5 (Interleukin-5)（Suspended beyond orders placed 03-29-2019）	Serum 0.5	S09 ↓ A00			Please confirm in advance	ELISA ELISA (Enzyme-Linked immunosorbent assay) A method in which after reacting an antigen with a solid-phase antibody, an enzyme-labeled antibody is reacted with the antigen in a secondary reaction, and the enzyme activity is measured by adding a chromogenic substrate.	3.9 or less (Reference value)(pg/mL)

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