TEST DIRECTORY

AND OR

Laboratory：Hachioji

○cytokines

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Soluble IL-2 receptor (sIL-2R)	serum 0.5	S09 ↓ A00		（21 days）	2-4	CLEIA Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.	157-474 (U/mL)
Th2 chemokine (TARC)	serum 0.3	S09 ↓ A00			2-4	CLEIA Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.	Child; 6-12 months : < 1367 Child; 1-2 years old : < 998 Child; ≧ 2 years old : < 743 Adults: < 450 (pg/mL)
Human interferon γ, EIA	serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	≦ 0.1 (Reference) (IU/mL)
Interleukin-1β (IL-1β)	serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	ELISA Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.	≦ 10 (Reference) (pg/mL)
Interleukin-4 (IL-4) (High sensitivity)	serum 0.3	S09 ↓ A00		（1 month）	Please contact us in advance.	CLEIA Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.	≦ 6.0 (Reference) (pg/mL)
Interleukin-6 (IL-6)	serum 0.5	S09 ↓ A00		（28 days）	2-5	CLEIA Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.	≦ 4.0 (pg/mL)
Interleukin-8 (IL-8)	culture fluid 0.5	A00			Please contact us in advance.	ELISA Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.	(pg/mL)
Interleukin-8 (IL-8)	plasma 0.5	PN2，PN5 ↓ A00			Please contact us in advance.	ELISA Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.	≦ 2.0 (Reference) (pg/mL)
Interleukin-10 (IL-10)	serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	ELISA Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.	≦ 5 (Reference) (pg/mL)
Transforming growth factor β1 (TGF-β1)	plasma 0.5	PN2，PN5 ↓ A00		（1 month）	Please contact us in advance.	ELISA Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.	1.56-3.24 (Reference) (ng/mL)
Granulocyte-colony stimulating factor (G-CSF)	serum 0.5	S09 ↓ A00		（1 month）	Please contact us in advance.	ELISA Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.	≦ 39.0 (Reference) (pg/mL)