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gene-related test
genetic tests related to transplantation

Laboratory：Hachioji

○genetic tests related to transplantation

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
HLA-A, B, serological typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			3-5	PCR-rSSO
HLA-A, DNA typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SBT
HLA-B, DNA typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SBT
HLA-C, DNA typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SBT
HLA-DR, serological typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			3-5	PCR-rSSO
HLA-DRB1, DNA typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SBT
HLA-DPB1, DNA typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SBT
HLA-DQA1, DNA typing（Suspended beyond orders placed 03-31-2021）	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SSP
HLA-DQB1, DNA typing	whole blood (with EDTA-2Na) 2.0	PN2,PN5			6-7	PCR-SBT
Chimerism analysis, pretransplant, recipient, PCR	whole blood (with EDTA-2Na) 7.0	PN7			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Chimerism analysis, pretransplant, recipient, PCR	bone marrow fluid 1.0	H00			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Chimerism analysis, pretransplant, donor, PCR	whole blood (with EDTA-2Na) 7.0	PN7			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Chimerism analysis, pretransplant, donor, PCR	bone marrow fluid 1.0	H00			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Chimerism analysis, pretransplant, donor, PCR	umbilical cord blood 1.0	H00			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Chimerism analysis, posttransplant, PCR	bone marrow fluid 1.0	H00			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Chimerism analysis, posttransplant, PCR	whole blood (with EDTA-2Na) 7.0	PN7			4-6	PCR Polymerase chain reaction (PCR) A technique to amplify a specific target region of DNA in an exponential manner. By heating DNA molecules, the DNA denatures and becomes single stranded, and then by cooling, the DNA anneals to the double stranded again. By using this phenomenon, a single-stranded DNA is used as a template to bind the target primer. The DNA synthesis occurs by repeated cycles of DNA polymerase activity.
Anti-HLA antibody, screening	serum 1.0	S09 ↓ A00		（26 days）	3-6	Luminex
Flow cytometry crossmatch, lymphocyte crossmatch test	(Recipient) whole blood and (Donor) whole blood (with ACD-A) (Recipient) 20.0 and (Donor) 7.5	S7P and PAR		（2 days）	3-5	Flow cytometry	B:<2.00 T:<1.50