High performance liquid chromatography (HPLC) A chromatography technique using a liquid mobile phase. HPLC separates a mixture into components promptly and accurately, using a column filled with a high-density absorbent and a high-pressure pump.
Nitrite ion: ≦ 1 Nitrate ion: 10-71 (μmol/L)
PCSK9(Suspended beyond orders placed 03-31-2021)
serum
0.3
S09 ↓ A00
4-10
ELISA
Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.
(ng/mL)
Leptin
serum
0.5
S09 ↓ A00
3-9
RIA2 antibody assay
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
(ng/mL)
High molecular weight adiponectin, CLEIA
serum
0.3
S09 ↓ A00
(28 days)
2-8
CLEIA
Chemiluminescent enzyme immunoassay (CLEIA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chemiluminescent substrate, the emission intensity is measured.
Electro chemiluminescence immunoassay (ECLIA) Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.
See below.
Active glucagonlike peptide-1, extraction(Suspended beyond orders placed 12-03-2020)
plasma(adding DPP-IV inhibitor at the time of blood sampling)
0.5
PAP ↓ A00
(2 months)
Please contact us in advance.
Pretreatment:Solid-phase extraction Assay:ELISA
(pmol/L)
Active glucosedependent insulinotropic polypeptide, extraction(Suspended beyond orders placed 12-03-2020)
plasma(adding DPP-IV inhibitor at the time of blood sampling)
0.5
plasma(adding DPP-IV inhibitor at the time of blood sampling)
0.5
PAP ↓ A00
(28 days)
Please contact us in advance.
ELISA
Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.
(pmol/L)
DNA histogram (frozen)(Suspended beyond orders placed 12-03-2020)
tissues
≧1.0g
A00
(-70℃)
Please contact us in advance.
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.
Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.
plasma(adding DPP-IV inhibitor at the time of blood sampling)
0.5
PAP ↓ A00
(28 days)
Please contact us in advance.
ELISA
Enzyme-linked immunosorbent assay (ELISA) Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.
Radio immunoassay (RIA) A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity. To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.
Concentration (ng/mL) Converted to (μg/g CRE)
DNA histogram(Suspended beyond orders placed 12-03-2020)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
(%)
DNA index(Suspended beyond orders placed 12-03-2020)
peripheral blood (with heparin)
10.0
PH5
Please contact us in advance.
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
DNA histogram(Suspended beyond orders placed 12-03-2020)
bone marrow fluid
1×107cells
H00
Please contact us in advance.
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
(%)
DNA histogram(Suspended beyond orders placed 12-03-2020)
peripheral blood (with heparin)
10.0
PH5
Please contact us in advance.
Flow cytometry(FCM)
Flow cytometry A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen). Two-color flow cytometry uses a combination of fluorochromes for double staining.
Notifications of URL changes/lab information added
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Notifications of URL changes/lab information added
You can now view test items from all labs.
Please choose a lab from the list below.
You can switch between labs as any time using the upper right lab icon.
The domain name of the TEST DIRECTORYpage has changed.
Please update bookmarks and saved links with the new address.