TEST DIRECTORY

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Laboratory：Hachioji

○plasma proteins

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

Prealbumin

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Turbidimetric immunoassay(TIA)

Turbidimetric immunoassay (TIA)
A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 22.0-40.0
(mg/dL)

α1-Microglobulin

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay Male: 12.5-25.5
Female: 11.0-
19.0
(mg/L)

α1-Microglobulin

random urine
0.5
U00 Refrigeration
 2-4 Latex agglutination immunoassay Male: 1.0-15.5
Female: 0.5-9.5
(mg/L)

α1-Antitrypsin

serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 94-150
(mg/dL)

α1-acidglycoprotein

serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 Male: 45-98
Female: 39-86
(mg/dL)

Retinol binding protein (RBP)

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay

Latex agglutination immunoassay (LA)
Using antigen- or antibody-coated latex particles, the antigen-antibody reaction is carried out. The turbidity of clots formed by the antigen-antibody reaction is measured by the light transmittancy or light scattering intensity.

 Male: 2.7-6.0
Female: 1.9-4.6
(mg/dL)

α2-macroglobulin

serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 Male: 100-200
Female: 130-250
(mg/dL)

Haptoglobin

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 Type 1-1: 83-
209
Type 2-1: 66-
218
Type 2-2: 25-
176
(mg/dL)

Ceruloplasmin

serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 21-37
(mg/dL)

Transferrin

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Turbidimetric immunoassay(TIA)

Turbidimetric immunoassay (TIA)
A technique to determine the light transmittancy by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 Male: 190-300
Female: 200-340
(mg/dL)

Transferrin, Urine

urine collected for 24 hrs
0.5
U00 Refrigeration
（1 month）
 2-4 Latex agglutination immunoassay

Latex agglutination immunoassay (LA)
Using antigen- or antibody-coated latex particles, the antigen-antibody reaction is carried out. The turbidity of clots formed by the antigen-antibody reaction is measured by the light transmittancy or light scattering intensity.

 ≦ 0.80
(mg/day)

Transferrin, Urine (creatinine ratio)

random urine
1
U00 Refrigeration
（1 month）
 2-4 Latex agglutination immunoassay

Latex agglutination immunoassay (LA)
Using antigen- or antibody-coated latex particles, the antigen-antibody reaction is carried out. The turbidity of clots formed by the antigen-antibody reaction is measured by the light transmittancy or light scattering intensity.

 Transferrin, Urine
adjustment
≦ 1.00
(mg/g CRE)

β2-microglobulin

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay 1.0-1.9
(mg/L)

β2-microglobulin

urine collected for 24 hrs
0.5
U00 Refrigeration
 2-4 Latex agglutination immunoassay ≦ 230
(μg/L)

β2-microglobulin

random urine
0.5
U00 Refrigeration
 2-4 Latex agglutination immunoassay ≦ 230
(μg/L)

C-reactive protein (CRP) quantitative

serum
0.6
S09
↓
A00 		Refrigeration
 2-4 Latex turbidimetric immunoassay ≦ 0.30
(mg/dL)

Pentraxin 3 (PTX3)

Immediately Refrigerated
plasma
0.3
PN2,PN5
↓
A00 		Freeze
 3-9 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 0.73-5.49(ng/mL)

Amyloid A protein, Serum (SAA)

serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Latex agglutination immunoassay ≦ 8.0
(μg/mL)

Myoglobin

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLIA

Chemiluminescent immunoassay (CLIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with chemiluminescentlabeled antibodies. The emission intensity of chemiluminescent substance is measured.

 Male: ≦ 154.9
Female: ≦ 106.0
(ng/mL)

Myoglobin, Urine

random urine
6
U50 Refrigeration
（21 days）
 2-5 RIA2 antibody assay

Radio immunoassay (RIA)
A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity.
　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.

 ≦ 10
(ng/mL)

Human heart type fatty acid-binding protein (H-FABP)

serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Latex agglutination method < 6.2
(ng/mL)

Ventricle myosin light chain I

serum
0.5
S09
↓
A00 		Refrigeration
 2-5 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 ≦ 2.5
(ng/mL)

Myocardial troponin T

serum
0.5
S09
↓
A00 		Refrigeration
（14 days）
 2-4 ECLIA

Electro chemiluminescence immunoassay (ECLIA)
Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.

 ≦ 0.014
Cutoff to
diagnose acute
myocardial
infarction
0.100
(ng/mL)

Ferritin

serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 Male: 39.4-340
Female: 3.6-114
(ng/mL)

L fatty acid binding protein (L-FABP), CLEIA

random urine
2.0
U00 Refrigeration
（14 days）
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 L-FABP
creatinine ratio:
≦ 8.4
(μg/gCr)

Urine NGAL

random urine
2.0

↓
U00 		Refrigeration
（7 days）
 2-4 CLIA

Chemiluminescent immunoassay (CLIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with chemiluminescentlabeled antibodies. The emission intensity of chemiluminescent substance is measured.

 ≦ 30.5 (ng/mL)
Creatinine ratio:
≦ 21.7 (μg/gCr)

Human oncofetal fibronectin

vaginal secretion
VP5 Freeze
（1 month）
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Negative

Procollagen type I N-propeptide (total P1NP)

serum
0.4
S09
↓
A00 		Refrigeration
（28 days）
 2-4 ECLIA

Electro chemiluminescence immunoassay (ECLIA)
Antibody-bound beads are reacted with antigens that are secondarily reacted with antibodies labeled with rutheniumpyridine complexes, which promotes the electrochemical reaction. The emission intensity of ruthenium-pyridine complexes is measured.

 Male
age 30-83: 18.1-74.1
Premenopausal
age 30-44: 16.8-70.1
Postmenopausal
age 45-79: 26.4-98.2
(ng/mL)

Collagen type I cross-linked N-telopeptide (NTx)

random urine
3
U00 Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 See below.

Collagen type I cross-linked N-telopeptide (NTx), osteoporos

random urine
3
U00 Refrigeration
 2-4 ELISA

Enzyme-linked immunosorbent assay (ELISA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies. After adding a chromogenic substrate, the enzyme activity is measured.

 Male : 13.0-66.2
Premenopausal: 9.3-54.3
Postmenopausal: 14.3-89.0
(Reference)
(nmolBCE/mmol CRE)
See the following criteria.

Collagen type I, C-terminal telopeptide (ICTP)

serum
0.3
S09
↓
A00 		Refrigeration
 2-4 RIA2 antibody assay

Radio immunoassay (RIA)
A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity.
　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.

 < 4.5(ng/mL)

Mac-2 binding protein glycosylated isomers (M2BPGi)

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 (-)
See below.

Autotaxin

serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 FEIA

Fluorescence enzyme immunoassay (FEIA)
One of the techniques of EIA. Using either enzyme-labeled antigen or antibody as a labeled-substance, the antigenantibody reaction is carried out. After adding a fluorescence substrate, the fluorescence intensity is measured.

 See below.
(mg/L)

Procollagen III peptide (P-III-P), CLIA

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLIA

Chemiluminescent immunoassay (CLIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with chemiluminescentlabeled antibodies. The emission intensity of chemiluminescent substance is measured.

 3.62-9.52
(ng/mL)

Collagen type IV

serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay

Latex agglutination immunoassay (LA)
Using antigen- or antibody-coated latex particles, the antigen-antibody reaction is carried out. The turbidity of clots formed by the antigen-antibody reaction is measured by the light transmittancy or light scattering intensity.

 ≦ 150
(ng/mL)

Collagen type IV, Urine

random urine
5
U70 Refrigeration
（1 month）
 3-9 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 30-39 years old
≦ 4.0
≧ 40 years old
≦ 4.9
(μg/g CRE)

Deoxypyridinoline

random urine
3
U00 Refrigeration
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 Creatinine adjustment
Male: 2.1-5.4
Female: 2.8-7.6
(nmol/mmol CRE)

Deoxypyridinoline (DPD), osteoporosis

random urine
3
U00 Refrigeration
 2-4 EIA

Enzyme immunoassay (EIA)
EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.

 Creatinine ratio
Male: 2.1-5.4
Female: 2.8-7.6
(nmol/mmol CRE)
See the following criteria.

INACTIVE
Eosinophil cationic protein (ECP)（Suspended beyond orders placed 03-31-2021）

serum
0.2
SEC
↓
A00 		Refrigeration
 2-8 FEIA

Fluorescence enzyme immunoassay (FEIA)
One of the techniques of EIA. Using either enzyme-labeled antigen or antibody as a labeled-substance, the antigenantibody reaction is carried out. After adding a fluorescence substrate, the fluorescence intensity is measured.

 Adult: ≦ 14.9
(μg/L)

KL-6

serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 < 500
(U/mL)

Procalcitonin

serum
0.4
S09
↓
A00 		Freeze
（28 days）
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 ≦ 0.05
(ng/mL)

Presepsin

Immediately
serum
0.4
S09
↓
A00 		Freeze
（28 days）
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 The cutoff value
for the diagnosis
of sepsis
(bacterial):
500
（pg/mL）

Presepsin

Immediately
plasma
0.4
PH5
↓
A00 		Freeze
（28 days）
 2-4 CLEIA

Chemiluminescent enzyme immunoassay (CLEIA)
Antibodies linked to the solid phase are reacted with antigens that are secondarily reacted with enzyme-labeled antibodies.
After adding a chemiluminescent substrate, the emission intensity is measured.

 The cutoff value
for the diagnosis
of sepsis
(bacterial):
500
（pg/mL）

INACTIVE
Collagen type IV / 7S（Suspended beyond orders placed 03-31-2021）

serum
0.5
S09
↓
A00 		Refrigeration
 3-5 RIA2 antibody assay

Radio immunoassay (RIA)
A target antigen is labeled with radioactive isotope (RI) and bound to its specific antibodies which is then competitively reacted with an antigen in a specimen. After the antigen-antibody reaction, the bound labeled antigens (bound) are separated from the unbound ones (free), and the antigen concentration is determined based on the proportion of radioactivity.
　To separate the bound and free antigens (B/F separation), the following methods are used. Solid-phase method:Antibodies are linked to the solid phase. Double antibody method: Antigen-antibody complexes are bound to the second antibody and precipitated. Ammonium sulfate precipitation method: Antigen-antibody complexes are precipitated by ammonium sulfate. PEG method: Antigenantibody complexes are precipitated by a precipitation reagent.

 ≦ 6
(ng/mL)

INACTIVE
CDT/transferrin ratio（Suspended beyond orders placed 12-05-2019）

serum
0.6
S09
↓
A00 		Refrigeration
（21 days）
 Please contact us in advance. CDT:Nephelometry Transferrin:Turbidimetric Immunoassay(TIA) %CDT
1.24-2.16
Cutoff of
alcoholic liver
disease: 2.00
(%)
Transferrin
Male: 190-300
Female: 200-340
(mg/dL)

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