TEST DIRECTORY

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Laboratory：Hachioji

○plasma proteins

TEST NAME

 SPECIMEN
REQUIREMENT
（ｍL）		 CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY)		 TURNAROUND
TIME (DAY)		 METHODOLOGY REFERENCE RANGE
(UNIT)

Prealbumin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 22.0～40.0(mg/dL)

α1-Microglobulin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay M12.5～25.5
F11.0～19.0(mg/L)

α1-Microglobulin

Partial urine
0.5
U00 Refrigeration
 2-4 Latex agglutination immunoassay M1.0～15.5
F0.5～9.5(mg/L)

α1-Antitrypsin

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 94～150(mg/dL)

α1-acidglycoprotein

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 M45～98
F39～86(mg/dL)

Retinol binding protein (RBP)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination turbidimetry

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 M2.7～6.0
F1.9～4.6(mg/dL)

α2-macroglobulin

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 M100～200
F130～250(mg/dL)

Haptoglobin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 Type 1-1 83~209
Type 2-1 66~218
Type 2-2 25~176 (mg/dL)

Ceruloplasmin

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Nephelometry

Nephelometry
A technique to determine the light-scattering intensity by irradiating light to a turbid substance formed by an antigen-antibody reaction.

 21～37(mg/dL)

Transferrin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 immunoturbidimetric method

TIA (Turbidimetric immnoassay)
Immunoturbidimetry
A method in which light is irradiated onto a turbid substance caused by an antigen-antibody reaction and the transmittance is measured.

 M190～300
F200～340(mg/dL)

Urinary transferrin

Urine storage
0.5
U00 Refrigeration
（1 month）
 2-4 Latex agglutination turbidimetry

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 0.80 or less (mg/day)

Urinary transferrin (creatinine equivalent)

Partial urine
1
U00 Refrigeration
（1 month）
 2-4 Latex agglutination turbidimetry

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 Urinary transferrin equivalent value 1.00 or less (mg/g・CRE)

β2-microglobulin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination immunoassay 1.0～1.9(mg/L)

β2-microglobulin

Urine storage
0.5
U00 Refrigeration
 2-4 Latex agglutination immunoassay 230 or less (μg/L)

β2-microglobulin

Partial urine
0.5
U00 Refrigeration
 2-4 Latex agglutination immunoassay 230 or less (μg/L)

C-reactive protein (CRP) quantification

Serum
0.6
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination turbidimetry

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 0.14 or less (mg/dL)

Pentraxin 3 (PTX3)

Immediately Refrigerated
Plasma
0.3
PN2,PN5
↓
A00 		Freeze
 3-9 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 0.73～5.49(ng/mL)

Serum amyloid A protein (SAA)

Serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 Latex agglutination immunoassay 3.0 or less (mg/L)

Myoglobin

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
Chemiluminescent immunoassay method
After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.

 Male: 154.9 or less
Female: 106.0 or less
(ng/mL)

Urinary myoglobin 〔CLEIA〕

Partial urine
6
U50 Refrigeration
（22 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 2.0(ng/mL)

Fatty acid binding protein from human heart (H-FABP)

Serum
0.5
S09
↓
A00 		Refrigeration
（28 days）
 2-4 latex agglutination method Less than 6.2 (ng/mL)

Ventricular myosin light chain I

Serum
0.5
S09
↓
A00 		Refrigeration
 2-6 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 2.5 or less (ng/mL)

Myocardial troponin T

Serum
0.5
S09
↓
A00 		Refrigeration
（14 days）
 2-4 ECLIA

ECLIA (Electro chemiluminescence immunoassay)
Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.

 0.014 or less
Cutoff value for acute myocardial infarction diagnosis
0.100 (ng/mL)

Ferritin

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 M39.4～340F3.6～114(ng/mL)

L-fatty acid binding protein (L-FABP)〔CLEIA〕

Partial urine
2
U00 Refrigeration
（14 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 L-FABP creatinine equivalent value 8.4 or less (μg/gCr)

Urinary NGAL

Partial urine
2

↓
U00 		Refrigeration
（7 days）
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
Chemiluminescent immunoassay method
After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.

 30.5 or less (ng/mL)
Creatinine equivalent value 21.7 or less (μg/gCr)

Fetal human cancer fibronectin

vaginal secretions
VP5 Freeze
（1 month）
 2-4 ELISA

ELISA (Enzyme-Linked immunosorbent assay)
Enzyme-linked immunosorbent assay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is reacted with the antigen secondarily, and a chromogenic substrate is added. A method of measuring enzyme activity.

 Negative

Type I procollagen-N-propeptide (total P1NP)

Serum
0.4
S09
↓
A00 		Refrigeration
（28 days）
 2-4 ECLIA

ECLIA (Electro chemiluminescence immunoassay)
Electro chemiluminescence immunoassay method . A method to measure the luminescence intensity of a ruthenium pyridine complex by reaction.

 Men (30-83 years old) 18.1-74.1
Premenopausal women (30-44 years old) 16.8-70.1
Postmenopausal women (45-79 years old) 26.4-98.2 (ng/mL)

Type I collagen cross-linked N-telopeptide (NTx)

Partial urine
3
U00 Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 See below

Type I collagen cross-linked N-telopeptide (NTx) (osteoporosis)

Partial urine
3
U00 Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Male 13.0~66.2
Premenopausal female 9.3~54.3
Postmenopausal female 14.3~89.0
(Reference value)
(nmolBCE/mmol・CRE) Judgment criteria: See below

Type I collagen C-terminal telopeptide (I CTP)

Serum
0.3
S09
↓
A00 		Refrigeration
 2-4 RIA2 antibody method

RIA (Radio immunoassay)
Radioimmunoassay
A competitive antigen-antibody reaction is performed between an antigen labeled with a radioisotope (RI) against an antibody and the antigen in the sample, and the labeled antigen bound to the antibody is A method in which labeled antigen (bound) and labeled antigen that is not bound to the antibody (free) are separated, and the ratio is measured as the antigen concentration from radioactivity.
Methods for separating bound and free forms (B/F separation) include the solid phase method in which antibodies are immobilized, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, and the antigen-antibody complex Examples include the ammonium sulfate salting out method in which the complex is precipitated with ammonium sulfate, and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

 Less than 4.5 (ng/mL)

Mac-2 binding protein glycosylation isomer (M2BPGi)

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 (-) refer to the following

Autotaxin

Serum
0.5
S09
↓
A00 		Refrigeration
（21 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 See below.(mg/L)

Procollagen III peptide (P-III-P)〔CLIA〕

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLIA

CLIA (Chemiluminescent immunoassay)
Chemiluminescent immunoassay method
After reacting the antigen with the immobilized antibody, the antibody labeled with a chemiluminescent substance is caused to react with the antigen for a second time to generate chemiluminescent A method for measuring the luminescence intensity of substances.

 3.62～9.52(ng/mL)

Collagen type IV

Serum
0.4
S09
↓
A00 		Refrigeration
 2-4 Latex agglutination turbidimetry

LA (Latex agglutination immunoassay)
Latex agglutination turbidimetry
An antigen-antibody reaction is performed using latex particles that have adsorbed (bound) antigens or antibodies, and the turbidity of the agglutination caused by the antigen-antibody reaction is measured using light. A method of measuring from transmittance or scattering intensity of light by irradiation.

 150 or less (ng/mL)

Urinary type IV collagen

Partial urine
5
U70 Refrigeration
（1 month）
 3-9 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 30-39 years old: 4.0 or less
40 years or older: 4.9 or less (μg/g・CRE)

Collagen type Ⅳ/ 7S〔CLEIA〕

Serum
0.5
S09
↓
A00 		Refrigeration
（23 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 4.4 or less (ng/mL)

Deoxypyridinoline

Partial urine
3
U00 Refrigeration
 2-4 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 Creatinine conversion value
M 2.1 ~ 5.4
F 2.8 ~ 7.6 (nmol/mmol・CRE)

Deoxypyridinoline (DPD) (osteoporosis)

Partial urine
3
U00 Refrigeration
 2-4 Enzyme immunoassay(EIA)

EIA (Enzyme immunoassay)
Enzyme immunoassay
The measurement principle is the same as RIA; an antigen-antibody reaction is performed using an enzyme-labeled antigen or antibody as a labeling substance, and a chromogenic substrate is added to detect enzyme activity. How to measure.

 Creatinine conversion ratio
M 2.1~5.4
F 2.8~7.6 (nmol/mmol・CRE)
Judgment criteria: See below

KL-6

Serum
0.5
S09
↓
A00 		Refrigeration
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Less than 500 (U/mL)

Procalcitonin

Serum
0.4
S09
↓
A00 		Freeze
（28 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 0.05 or less (ng/mL)

Presepsin

Immediately
Serum
0.4
S09
↓
A00 		Freeze
（28 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Cutoff value for sepsis (bacterial) diagnosis
500 (pg/mL)

Presepsin

Immediately
Plasma
0.4
PH5
↓
A00 		Freeze
（28 days）
 2-4 CLEIA

CLEIA (Chemiluminescent enzyme immunoassay)
Chemiluminescent enzyme immunoassay
After reacting the antigen with the immobilized antibody, the enzyme-labeled antibody is subjected to a secondary reaction with the antigen, and a chemiluminescent substrate is added. method to measure luminescence intensity.

 Cutoff value for sepsis (bacterial) diagnosis
500 (pg/mL)

INACTIVE
Urinary myoglobin（Suspended beyond orders placed 03-31-2022）

Partial urine
6.0
U50 Refrigeration
（21 days）
 2-5 RIA2 antibody method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

 10 or less (ng/mL)

INACTIVE
CDT/transferrin ratio（Suspended beyond orders placed 12-05-2019）

Serum
0.6
S09
↓
A00 		Refrigeration
（21 days）
 Please confirm in advance CDT: Nephelometry
Transferrin: immunonephelometry 		%CDT
1.24-2.16
Cutoff value for alcoholic liver damage 2.00
(%)

Transferrin
M 190-300
F 200-340
(mg/dL)

INACTIVE
ECP（Suspended beyond orders placed 03-31-2021）

Serum
0.2
SEC
↓
A00 		Refrigeration
 2-8 FEIA

FEIA (Fluorescence enzyme immunoassay)
Fluorescence enzyme immunoassay
A method of EIA in which enzyme-labeled antigen or antibody is used for antigen-antibody reaction and fluorescent substrate is added for the detection

 Adult 14.9 or less (μg/L)

INACTIVE
Type IV Colagen 7S（Suspended beyond orders placed 03-31-2021）

Serum
0.5
S09
↓
A00 		Refrigeration
 3-5 RIA2 antibody method

RIA (Radio immunoassay)
Radioimmunoassay
A method to perform the competitive antigen-antibody reaction between the antigen labeled with radioisotope (RI) against the antibody and the antigen in the specimen, and to separate the labeled antigen bound to the antibody (bound type: Bound) from the labeled antigen not bound to the antibody (free type: Free) and to measure the ratio of them as the antigen concentration from the radioactivity.
As separation methods for bound and free types (B/F separation), there are the solid-phase method in which the antibody is solid-phased, the two-antibody method in which a second antibody is bound to the antigen-antibody complex and precipitated, the ammonium sulfate precipitation method in which the antigen-antibody complex is precipitated with ammonium sulfate (ammonium sulfate), and the PEG method in which the antigen-antibody complex is precipitated with a precipitation reagent.

 6 or less (ng/mL)

INACTIVE
Pro-collagen Ⅲ peptide (P-Ⅲ-P)（Suspended beyond orders placed 01-06-2017）

Serum
0.3
S09
↓
A00 		Refrigeration
 2-4 RIA solid phase method

IRMA (Immuno radio metric assay)
Immuno radiometric assay
One of the RIA methods in which an antigen is reacted with a solid-phase antibody, followed by a secondary reaction of the antigen with a radioisotope (RI)-labeled antibody.
This method is also called the sandwich method because a solid-phase antibody and a labeled antibody bind to each other by sandwiching the antigen.

 0.3-0.8(U/mL)

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