TEST DIRECTORY

AND OR

TOP
biochemical test
organic acids

Laboratory：Hachioji

○organic acids

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
Lactic acid	deproteinized supernatant 0.4	M30 ↓ A00			2-4	Enzymatic assay of lactate oxidase Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	3.0-17.0 (whole blood) (mg/dL)
Pyruvic acid	deproteinized supernatant 0.4	M30 ↓ A00			2-4	Enzymatic assay of pyruvate oxidase Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	0.30-0.94 (whole blood) (mg/dL)
Oxalic acid, Urine	aliquot of acidified 24 hrs. urine 3	A00		（21 days）	3-9	Capillary electrophoresis Electrophoresis A technique for analysis of macromolecules based on the following phenomena: When an electric current is applied to an electrolyte solution in which charged particles suspended, the particles migrate towards the electrode of opposite charge. Based on the length of migration, the target molecule is determined. Cellulose acetate membrane, agarose gel, and polyacrylamide gel are used as support media.	Male: 10.3-41.5 Female: 9.0-37.7 (mg/day)
Citric acid	random urine 0.5	U00		（1 month）	6-12	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	138-1010 (mg/L)
Citric acid	serum 1.5	S09 ↓ A00			6-12	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	1.3-2.6 (mg/dL)
Ketone bodies fractionation	serum (vein) 0.5	S09 ↓ A00		（7 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	Acetoacetic acid ≦ 55 3-hydroxy butyric acid ≦ 85 Total ketone bodies ≦ 130 (μmol/L)
Ketone bodies fractionation	serum (artery) 0.5	S09 ↓ A00		（7 days）	2-4	Enzymatic method Enzymatic method This technique is based on the same assay principle as the colorimetry. Analytes are specifically measured using enzymatic reactions.	Ketone bodies ratio Acetoacetic acid/ 3-hydroxy butyric acid ratio ≧ 0.7
Acetone, quantitative	serum 0.7	S7P ↓ A00		（15 days）	7-10	Gas chromatography Chromatography A technique for separation of components of a mixture by passing it in a flowing mobile phase (liquid or gas) that passes a stationary phase (solid or liquid). The difference in the affinity of the components to the two phases is used for separation. In liquid chromatography, the mobile phase is a liquid, whereas in gas chromatography, the mobile phase is a gas.	< 5 (μg/mL)
Acetone, Urine, quantitative	random urine 1.2	U00			7-10	Gas chromatography Chromatography A technique for separation of components of a mixture by passing it in a flowing mobile phase (liquid or gas) that passes a stationary phase (solid or liquid). The difference in the affinity of the components to the two phases is used for separation. In liquid chromatography, the mobile phase is a liquid, whereas in gas chromatography, the mobile phase is a gas.	< 5 (μg/mL)
Organic acid-screening	random urine 5	U00			7-14	Gas chromatography(GC-MS) Chromatography A technique for separation of components of a mixture by passing it in a flowing mobile phase (liquid or gas) that passes a stationary phase (solid or liquid). The difference in the affinity of the components to the two phases is used for separation. In liquid chromatography, the mobile phase is a liquid, whereas in gas chromatography, the mobile phase is a gas.