antiepileptic drug | TEST DIRECTORY

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antiepileptic drug

Laboratory：Hachioji

○antiepileptic drug

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)	BLOOD COLLECTION TIME
Carbamazepine	serum 0.5	S7P ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	4-12 (μg/mL)	Immediately before the next administration(Trough concentration)
Carbamazepine	plasma 0.5	PH5 ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	4-12 (μg/mL)	Immediately before the next administration(Trough concentration)
Clonazepam	serum 0.3	S7P ↓ A00			3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	20-70 (ng/mL)	Immediately before the next administration(Trough concentration)
Diazepam	serum 0.5	S7P ↓ A00			3-5	HPLC High performance liquid chromatography (HPLC) A chromatography technique using a liquid mobile phase. HPLC separates a mixture into components promptly and accurately, using a column filled with a high-density absorbent and a high-pressure pump.	Diazepam 600-1000 (ng/mL)	Immediately before the next administration(Trough concentration) However, if it is used as an ant
Ethosuximide	serum 0.5	S7P ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	40.0-100.0 (μg/mL)	Immediately before the next administration(Trough concentration)
Ethosuximide	plasma 0.5	PH5 ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	40.0-100.0 (μg/mL)	Immediately before the next administration(Trough concentration)
Clobazam	serum 0.3	S7P ↓ A00			3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(ng/mL)
Nitrazepam	serum 0.3	S7P ↓ A00			3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	20-200 (ng/mL)	Immediately before the next administration(Trough concentration) However, if it is used as an ant
Phenobarbital	serum 0.5	S7P ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	10-40 (μg/mL)	Immediately before the next administration(Trough concentration)
Phenobarbital	plasma 0.5	PH5 ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	10-40 (μg/mL)	Immediately before the next administration(Trough concentration)
Phenytoin	serum 0.5	S7P ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	10.0-20.0 (μg/mL)	Oral:Immediately before the next administration(Trough concentration) Intravenous:Postdose2-4 hou
Phenytoin	plasma 0.5	PH5 ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	10.0-20.0 (μg/mL)	Oral:Immediately before the next administration(Trough concentration) Intravenous:Postdose2-4 hou
Free phenytoin	serum 1.0	S7P ↓ A00		（14 days）	4-10	Ultrafiltration EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	(μg/mL)	Oral:Immediately before the next administration(Trough concentration) Intravenous:Postdose2-4 hou
Primidone	serum 0.3	S7P ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	5.0-12.0 (μg/mL)	Immediately before the next administration(Trough concentration)
Primidone	plasma 0.3	PH5 ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	5.0-12.0 (μg/mL)	Immediately before the next administration(Trough concentration)
Valproate	serum 0.5	S7P ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	50-100 (μg/mL)	Immediately before the next administration(Trough concentration)
Valproate	plasma 0.5	PH5 ↓ A00			2-4	EIA Enzyme immunoassay (EIA) EIA is based on the same assay principle as RIA. Using either enzyme-labeled antigen or antibody as a labeledsubstance, the antigen-antibody reaction is carried out. After adding a chromogenic substrate, the enzyme activity is measured.	50-100 (μg/mL)	Immediately before the next administration(Trough concentration)
Free valproate	serum 1.0	S7P ↓ A00		（14 days）	4-10	Ultrafiltration EIA	(μg/mL)	Immediately before the next administration(Trough concentration)
Trimethadione	serum 0.5	S7P ↓ A00		（12 days）	7-13	LC-MS/MS	300-500 (μg/mL)
Zonisamide	serum 0.5	S7P ↓ A00			2-4	Latex agglutination method	10-30 (μg/mL)	Immediately before the next administration(Trough concentration)
Gabapentin	plasma 0.3	PH5 ↓ A00			3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(μg/mL)
Lamotrigine	plasma 0.3	PH5 ↓ A00			3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(μg/mL)
Topiramate	plasma 0.3	PH5 ↓ A00		（21 days）	3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(μg/mL)
Levetiracetam	plasma 0.3	PH5 ↓ A00		（21 days）	3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(μg/mL)	Immediately before the next administration(Trough concentration)
Perampanel	plasma 0.3	PH5 ↓ A00		（28 days）	3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(ng/mL)
Lacosamide	plasma 0.3	PH5 ↓ A00		（28 days）	3-5	LC/MS/MS Liquid chromatography-tandem mass spectrometry (LC/MS/MS) Liquid chromatography separates the target substance into compounds using difference in the affinity, which is combined with the mass spectrometry function that further divides the compounds by mass difference to isolate, fragment, and detect a specific mass ion.	(μg/mL)	Immediately before the next administration(Trough concentration)
Acetazolamide	serum 0.3	S7P ↓ A00			3-9	HPLC High performance liquid chromatography (HPLC) A chromatography technique using a liquid mobile phase. HPLC separates a mixture into components promptly and accurately, using a column filled with a high-density absorbent and a high-pressure pump.	(μg/mL)

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