Laboratory:Hachioji

Laboratory:Hachioji
TEST NAME |
SPECIMEN REQUIREMENT (mL) |
CONTAINER | CAP COLOR | STORE TEMPERATURE (STABILITY) |
TURNAROUND TIME (DAY) |
METHODOLOGY | REFERENCE RANGE (UNIT) |
---|---|---|---|---|---|---|---|
BRAF V600 mutation analysis, PCR
|
unstained slides
5-10 sildes thickness:10μm |
Z10 |
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4-10 |
PCR(Real-time PCR) Real-time PCR |
||
BRAF exon15 V600E (SEQ)
|
unstained slides
|
Z10 |
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11-14 |
Direct sequencing method Direct sequencing method |
||
EGFR mutation analysis v2.0
|
unstained slides
5-10 sildes thickness:10μm |
Z10 |
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3-6 |
PCR(Real-time PCR) Real-time PCR |
||
EGFR mutation analysis v2.0 (plasma)
|
plasma
5.0 |
PK5,PK7 ↓ ARR |
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3-6 |
PCR(Real-time PCR) Real-time PCR |
|
EGFR gene mutational analysis (Scorpion-ARMS technique)
|
unstained slides
5-10 sildes thickness:5-10μm |
Z10 |
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4-7 |
PCR(Real-time PCR) Real-time PCR |
||
|
tissues
100mg |
ARR |
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4-7 |
RT-PCR(Real-time PCR) Real-time PCR |
Negative | |
|
pleural fluid sediment
|
ARR |
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4-7 |
RT-PCR(Real-time PCR) Real-time PCR |
Negative | |
|
bronchoalveolar lavage fluid sediment
|
ARR |
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4-7 |
RT-PCR(Real-time PCR) Real-time PCR |
Negative | |
|
bronchial brushing lavage fluid sediment
|
ARR |
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4-7 |
RT-PCR(Real-time PCR) Real-time PCR |
Negative | |
Qualitative detection of ROS1 gene fusions (FFPE)
|
unstained paraffin sections
5paraffin sections |
ARR |
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4-7 |
RT-PCR(Real-time PCR) Real-time PCR |
Negative | |
Qualitative detection of ROS1 gene fusions (FFPE)
|
unstained slides
5 sildes thickness:5μm |
Z10 |
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4-7 |
RT-PCR(Real-time PCR) Real-time PCR |
Negative | |
|
pleural fluid sediment
|
ARR |
![]() (3 days) |
7-11 |
RT-PCR(Real-time PCR) Real-time PCR |
None-detected | |
|
bronchoalveolar lavage fluid sediment
|
ARR |
![]() (3 days) |
7-11 |
RT-PCR(Real-time PCR) Real-time PCR |
None-detected | |
|
bronchial brushing lavage fluid sediment
|
ARR |
![]() (3 days) |
7-11 |
RT-PCR(Real-time PCR) Real-time PCR |
None-detected | |
|
tissues
100mg |
ARR |
![]() (3 days) |
7-11 |
RT-PCR(Real-time PCR) Real-time PCR |
None-detected | |
PIK3CA mutation analysis, SEQ
|
unstained slides
|
Z10 |
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11-14 |
Direct sequencing method Direct sequencing method |
||
c-kit mutation analysis (GIST)
|
tissues
250mg |
ARR |
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11-23 |
Direct sequencing method Direct sequencing method |
||
c-kit mutation analysis (GIST)
|
unstained slides
|
Z10 |
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11-23 |
Direct sequencing method Direct sequencing method |
||
RAS/BRAF gene mutational analysis
|
unstained slides
5 sildes thickness:5-10μm |
Z10 |
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4-7 | PCR-rSSO | ||
Microsatellite instability (MSI) test (FFPE)
|
unstained slides
5-10 sildes thickness:5μm |
Z10 |
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4-8 |
Multiplex PCR-fragment analysis Multiplex PCR-fragment analysis |
||
Microsatellite instability (MSI) test (FF)
|
tissues
50mg |
ARR |
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4-8 |
Multiplex PCR-fragment analysis Multiplex PCR-fragment analysis |
||
IDH1/2 gene analysis (Glioma) (FFPE)
|
unstained slides
5–10 sildes thickness:4-10μm |
Z10 |
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11-14 |
Direct sequencing method ダイレクトシーケンス法 |
||
|
unstained slides
5-10 sildes thickness:5μm |
Z10 |
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11-14 |
Next-generation sequencing (NGS) method Next-generation sequencing (NGS) method |
||
|
tissues
100mg |
ARR |
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11-14 |
Next-generation sequencing (NGS) method Next-generation sequencing (NGS) method |
||
IDH1/2 gene analysis (Glioma) (FF)
|
tissues
125mg |
ARR |
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11-14 | Direct sequencing method | ||
|
unstained slides+whole blood
5 sildes thickness:10μm + 2.0 |
Z10+PK2 |
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16ー22 | Next generation sequence (NGS) method | |
|
unstained slides+slide for HE staining
10 sildes thickness:4-5μm + 1 slide for HE staining |
Z10 |
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16-19 | Next generation sequence (NGS) method | ||
|
100mg
|
ARR |
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7ー11 | Next generation sequence (NGS) method | ||
|
tissues
250mg |
ARR |
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12-16 |
Direct sequencing method Direct sequencing method |
||
|
unstained slides
5–10 sildes thickness:5μm |
Z10 |
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7-11 | Nextgeneration sequence(NGS)method | ||
|
tissues
100mg |
ARR |
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7-11 | Next generation sequence (NGS) method | ||
|
unstained slides
5–10 sildes thickness:5μm |
Z10 |
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7ー11 | Next generation sequence (NGS) method |