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gene-related test
genetic tests related to drug response

Laboratory：Hachioji

○genetic tests related to drug response

TEST NAME	SPECIMEN REQUIREMENT （ｍL）	CONTAINER	CAP COLOR	STORE TEMPERATURE (STABILITY)	TURNAROUND TIME (DAY)	METHODOLOGY	REFERENCE RANGE (UNIT)
IL28B SNPs analysis	whole blood (with EDTA-2Na) 5.0	PN5			12-16	PCR(Real-time PCR) and directsequencing method
Drug metabolizing enzyme cytochrome P450 CYP2C19 gene polymorphism analysis	whole blood (with EDTA-2Na) 2.0	PN2,PN5			12-16	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.
UGT1A1 gene polymorphism analysis	whole blood (with EDTA-2Na) 2.0	PN2，PN5			4-8	Invader method Invader method A technique consisting of two steps of homogeneous isothermal reaction to detect genetic polymorphisms by the use of cleavase (one of the endonucleases) that specifically recognizes and cleaves the triple-stranded DNA structure.
NUDT15 gene codon 139 polymorphism analysis	whole blood (with EDTA-2Na) 2.0	PN2，PN5		（3 days）	4-8	PCR(Real-time PCR) Real-time PCR Real-time PCR is the one of the nucleic acid amplification tests using polymerase chain reaction (PCR) as the basic principle. By the use of oligonucleotide that yields fluorescence after degradation, the fluorescent signal is identified for each PCR cycle, which enables the real-time quantification of the target nucleic acid.