MLC (mixed lymphocyte culture)

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cellular immunity test
cellular immunity test
MLC (mixed lymphocyte culture)

Laboratory：Hachioji

○MLC (mixed lymphocyte culture)

CODE：5178 0

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
MLC (mixed lymphocyte culture)
(Recipient) Blood (heparin addition) and
(Donor) Blood (heparin addition) and
(Unrelated person) Blood (heparin addition)

20.0, 10.0, and 10.0
PH9
9-11 ³H-thymidine uptake ability
³H-thymidine uptake ability (³H-TdR uptake) Method.
Lymphocytes are cultured with a stimulating substance and ³H-thymidine, and the amount of ³H-thymidine taken into the cells by DNA synthesis is expressed as radioactivity. Measure. PHA, ConA, drugs, etc. are used as stimulants.

COMMENT

Acceptable days are Tuesday to Friday. Please be sure to clearly state the relationship, clinical diagnosis name, administered drug name, blood transfusion history, and transplant history. MLC cultures lymphocytes under sterile conditions, so avoid opening the collection container or transferring the sample. MLC unrelated blood will be used as a control.
About MLC (Mixed Lymphocyte Culture)
MLC (Mixed Lymphocyte Culture) is a pre-booked test, so please be sure to contact our sales staff before blood collection.
Collect 20.0mL (2 bottles) of recipient blood, 10.0mL (1 bottle) of donor blood, and 10.0mL (1 bottle) of unrelated blood into the container shown below, and mix well. Please store at room temperature.
MLC cultures lymphocytes under sterile conditions, so avoid opening the collection container or transferring the sample.
Please write the facility name and name on the label of the container.
If the number of lymphocytes is low, please collect more blood.

CONTAINER

PH9 旧容器記号 I

ヘパリン入り (真空採血量10mL)

内容：ヘパリンNa　130IU
貯蔵方法：室温
有効期間：製造から2年

TEST DIRECTORY