Laboratory：Hachioji
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- Malignant lymphoma analysis test 7AAD analysis (hematopoietic organ malignant tumor cell test)
Laboratory：Hachioji
○Malignant lymphoma analysis test 7AAD analysis (hematopoietic organ malignant tumor cell test)
CODE：6202 3
-
TEST NAME
SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Malignant lymphoma analysis test 7AAD analysis (hematopoietic organ malignant tumor cell test)
lymph nodes
5×5×5mm
H20
（1 day）
3-5 Flow cytometry(FCM)Flow cytometry
A technique for analysis of individual cells. Cells stained with fluorochrome-labeled monoclonal antibodies are flown in a stream of solution at a high speed and are passed through the laser beam. The cells are individually analyzed by forward scatter (size of the cell), 90° side scatter (internal complexity of the cell), and fluorescence intensity (cell surface expression of the antigen).
Two-color flow cytometry uses a combination of fluorochromes for double staining.
COMMENT
Avoid freezing. Acceptable days are Monday through Friday. Please see below for the items to be performed. Avoid peripheral blood and bone marrow fluid.
About Requests for Cellular Immunity Tests
1. Submit the specimen on the same day after collection.
2. If the number of lymphocytes is low, collect extra amount of blood.
3. If you request with bone marrow fluid, please use the special container (H00) and submit it under refrigeration. However, since the CD34 quantitative cannot yield accurate test results if diluted with the preservation solution, please use a special container (PH5).
4. If you request with tissues such as lymph nodes, please submit them floated in a special container (H20) under refrigeration.
Float a 5 x 5 x 5 mm lymph node in the designated container and preserve it refrigerated. Submit the specimen on the same day after collection.
CONTAINER
H20 旧容器記号 H2
保存液入り (容器容量10mL)
内容：FBS PBS 硫酸カナマイシン
貯蔵方法：凍結
有効期間：製造から1年
supplementary information
白血病・リンパ腫解析検査 (LLA) ・悪性リンパ腫解析検査 (MLA)
多発性骨髄腫解析検査CD38マルチ解析
多発性骨髄腫マルチパラメーターフローサイトメトリー (8color)
悪性リンパ腫解析検査7AAD解析
| 項目 | 検出抗体 |
|---|---|
| 白血病・リンパ腫解析検査 (LLA) | CD1a CD2 CD3 CD4 CD5 CD7 CD8 CD10 CD13 CD14 CD19 CD20 CD33 CD34 CD41 CD56 GP-A HLA-DR |
| 悪性リンパ腫解析検査 (MLA) | CD2 CD3 CD4 CD5 CD7 CD8 CD10 CD11c CD16 CD19 CD20 CD23 CD25 CD30 CD34 CD56 κ-chain λ-chain |
| 多発性骨髄腫解析検査CD38マルチ解析 | CD7 CD19 CD20 CD33 CD45 CD49e CD54 CD56 CD138 MPC-1 cy κ-chain cy λ-chain |
| 多発性骨髄腫マルチパラメーターフローサイトメトリー (8color) | CD19 CD27 CD38※ CD45 CD56 CD138 cyIgκ cyIgλ |
| 悪性リンパ腫解析検査7AAD解析 | CD2 CD3 CD4 CD5 CD7 CD8 CD10 CD19 CD20 CD25 CD30 CD34 CD38 CD45 CD56 IgM κ-chain λ-chain |
※マルチエピトープ抗体を使用しております。
