EGFR mutation analysis v2.0 (plasma)

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gene-related test
genetic tests for malignant tumor (solid tumor)
EGFR mutation analysis v2.0 (plasma)

Laboratory：Hachioji

○EGFR mutation analysis v2.0 (plasma)

CODE：6350 0

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
EGFR mutation analysis v2.0 (plasma)
Plasma
5
PK5,PK7
↓
ARR
3-6 PCR (Real Time PCR)
Real-time PCR
A type of nucleic acid amplification method based on the basic principle of PCR, which uses oligonucleotides that emit fluorescence upon decomposition to quantify target nucleic acids in real time by checking the fluorescent signal at each PCR cycle. A measurement method that enables

COMMENT

●About plasma material: Collect a sufficient amount of blood using an EDTA-2K blood collection tube considering the amount of sample to be submitted (5 mL of plasma), and be sure to separate the plasma within 8 hours after blood collection. Immediately after separation, aliquot 2.5 mL of plasma into two sterile polyspits and store frozen. When collecting plasma, do not perform decantation to prevent contamination with genomic DNA derived from leukocyte components. Please avoid duplicate requests with other items. With this testing method, the affects of contamination are greater, so please be careful when handling the sample.
[6350 0] Notes on EGFR mutation analysis v2.0 (plasma) (from the reagent package insert)
1. In plasma samples, tumor-derived DNA does not leak sufficiently into the plasma. It is possible that the EGFR gene mutation cannot be detected even if it exists in the tumor tissue. Therefore, if a plasma test is performed first and a result of EGFR gene mutation is obtained, consider performing a tissue test if possible.
2. Plasma testing is not a complete replacement for tissue testing.
Please collect a sufficient amount of blood using the blood collection tube shown below considering the amount of sample to be submitted (5 mL of plasma), and be sure to separate the plasma within 4 hours after blood collection. Immediately after separation, aliquot 2.5 mL of plasma into two sterile polyspittes (ARR) and store frozen. When collecting plasma, do not perform decantation to prevent contamination with genomic DNA derived from leukocyte components.
Please avoid duplicate requests with other items. With this test method, the affects of contamination are greater, so please be careful when handling the sample.

CONTAINER

PK5

EDTA-2K入り (真空採血量5mL)

内容：EDTA-2K　9.5mg
貯蔵方法：室温
有効期間：製造から2年

PK7

EDTA-2K入り (真空採血量7mL)

内容：EDTA-2K　13.3mg
貯蔵方法：室温
有効期間：製造から2年

ARR 旧容器記号 r A

滅菌ポリスピッツ

貯蔵方法：室温

TEST DIRECTORY