Laboratory:Hachioji
- TOP
- gene-related test
- immune-relevant genetic rearrangement tests
- Immunoglobulin L chain Cλreconstitution
Laboratory:Hachioji
○Immunoglobulin L chain Cλreconstitution
CODE:2306 9
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TEST NAME
SPECIMEN
REQUIREMENT
(mL) CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT) -
Immunoglobulin L chain Cλreconstitution
Blood (EDTA-2Na added)
7
PN7
11-13 Southern Blot HybridizationGene rearrangement not recognizedSouthern Blot Hybridization
A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
COMMENT
Blood/bone marrow fluid/cells: Avoid cryopreservation. Please fill in the clinical diagnosis name etc. on the request form. Please submit the cells in a culture container (filled with culture medium) used in the hospital and keep them refrigerated. Tissues fixed with formalin, etc. cannot be analyzed by Southern blot hybridization because high-molecular DNA cannot be obtained.
Collect blood into the container shown below, mix well, and store in the refrigerator.
CONTAINER
PN7 旧容器記号 A 4
EDTA-2Na入り (真空採血量7mL)
内容:EDTA-2Na 10.5mg
貯蔵方法:室温
有効期間:製造から2年