Microsatellite instability (MSI) testing (Lynch syndrome)

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Microsatellite instability (MSI) testing (Lynch syndrome)

Laboratory：Hachioji

○Microsatellite instability (MSI) testing (Lynch syndrome)

CODE：3894 3

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
Microsatellite instability (MSI) testing (Lynch syndrome)
Unstained specimen slide
5 to 10 sheets
thickness 5?m
Z10
8-14 Multiplex PCR-fragment analysis
After amplifying multiple target genes, the PCR amplification products are subjected to capillary electrophoresis using a sequencer.
A method that separates DNA according to its size and analyzes and identifies peaks according to the amount of amplification using the QMVR width set by dedicated software.

COMMENT

Acceptable days are Monday to Friday.
We will analyze and judge 5 types of markers (BAT25, BAT26, NR21, NR24, MONO27). Please avoid duplicate requests with other items.
The percentage of tumor cells required for testing is more than 50%. Please see below for points to note when submitting unstained specimen slides.
●Submission conditions
Unstained specimen slides must be histopathologically evaluated to ensure that the proportion of tumor cells present is at least the amount required for testing. If the percentage of tumor cells is less than that, mark the tumor cell area from the back of the unstained specimen slide. Please note that if the sample is submitted without marking, macrodissection may not be possible and the results may be affected, such as false negatives.
In addition, due to the characteristics of the two items, microsatellite instability (MSI) testing (FFPE) and microsatellite instability (MSI) testing (Lynch syndrome), it is necessary to distinguish between tumor areas and normal areas. Please be sure to mark the tumor cell area.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation. .
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY