IDH1/2 gene analysis (glioma) (FFPE)

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gene-related test
genetic tests for malignant tumor (solid tumor)
IDH1/2 gene analysis (glioma) (FFPE)

Laboratory：Hachioji

○IDH1/2 gene analysis (glioma) (FFPE)

CODE：R617 0

TEST NAME SPECIMEN
REQUIREMENT
（ｍL） CONTAINER CAP COLOR STORE
TEMPERATURE
(STABILITY) TURNAROUND
TIME (DAY) METHODOLOGY REFERENCE RANGE
(UNIT)
IDH1/2 gene analysis (glioma) (FFPE)
Unstained specimen slide
5 to 10 sheets
thickness 4 to 10?m
Z10
11-14 Direct sequence method
Direct sequencing method
A method of directly determining the base sequence using DNA amplified by PCR method as a template.

COMMENT

￡　We are analyzing the gene mutations IDH1 R132 and IDH2 R172 in glioma. Please note that it is not possible to measure IDH2 gene mutations found in acute myeloid leukemia (AML) patients.
The percentage of tumor cells required for testing is 20% or more. Please see below for points to note when submitting unstained specimen slides.
Please avoid duplicate requests with other items. This testing method increases the influence of contamination, so please be careful when handling the specimen.
●Submission conditions
Unstained specimen slides must be histopathologically evaluated to ensure that the proportion of tumor cells present is at least the amount required for testing. If the percentage of tumor cells is less than that, mark the tumor cell area from the back of the unstained specimen slide. Please note that if the sample is submitted without marking, macrodissection may not be possible and the results may be affected, such as false negatives.
●About unstained specimen slides
Immediately immerse the collected tissue in a 10% neutral buffered formalin solution and fix it (recommended fixation time is 6 to 48 hours). When submitting, please prepare serial sections at the specified thickness from formalin-fixed paraffin-embedded (FFPE) blocks prepared within the last 3 years, if possible. Please be careful to avoid contamination by changing the microtome blade for each specimen when slicing. Please note that as nucleic acids are fragmented due to formalin fixation of tissues, analysis may not be possible depending on the type and composition of the fixative, fixation time, and storage condition of the specimen after fixation. .
●About biopsy specimens
Biopsy specimens often contain only a small amount of specimen, and there is a possibility that most of the tissue itself has disappeared or that the tissue has become a piece of tissue that does not contain tumor cells. Please be aware of this in advance.

CONTAINER

Z10 旧容器記号 t 30

[オブジェクトケース]
プレパラート (スライドグラス)

貯蔵方法：室温

TEST DIRECTORY