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Laboratory：Hachioji

○EB virus DNA (Clonality)

CODE：2494 7

  • TEST NAME SPECIMEN
    REQUIREMENT
    （ｍL）     CONTAINER CAP COLOR STORE
    TEMPERATURE
    (STABILITY)     TURNAROUND
    TIME (DAY)     METHODOLOGY REFERENCE RANGE
    (UNIT)
  • EB virus DNA (Clonality)
    Blood (EDTA-2Na added)
    7
    PN7 Refrigeration
    （10 days）
     17-23 Southern Blot Hybridization

    Southern Blot Hybridization
    A technique to detect a specific gene by the following procedures: After restriction enzyme digestion of DNA, DNA is isolated and denatured to a single-stranded form with electrophoresis. Using capillary action, the singlestranded DNA is transferred to a nylon membrane and hybridized with a target probe to detect the target gene. This technique is used for analysis of abnormal changes in DNA in a quantitative and qualitative manner.
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COMMENT


Blood: Avoid cryopreservation. Please note that if the number of cells has decreased due to chemotherapy, etc., it may not be possible to extract the required amount of DNA. Since high-molecular DNA cannot be obtained from tissues fixed with formalin, etc., it is impossible to analyze them by Southern blot hybridization. With this method, EBV DNA may not be detected if the amount of viral DNA is small.
Please note that if you request multiple samples at the same time, the number of days required may vary.
Collect blood into the container shown below, mix well, and store in the refrigerator.

CONTAINER

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